Rabbit anti-Mouse CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• Mast/stem cell growth factor receptor Kit, also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit, is encoded by the Kit (Sl) gene in mouse.
• Acts as a cell-surface receptor for the cytokine KITLG/SCF, playing essential roles in cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development and function, and melanogenesis.
• Upon KITLG/SCF binding, activates multiple signaling pathways including PI3K-AKT, RAS-MAPK, and JAK-STAT; phosphorylates PIK3R1, PLCG1, SH2B2, CBL, and recruits GRB2 leading to RAS/RAF/MAPK cascade activation.
• Belongs to the type III receptor tyrosine kinase family, sharing structural features with PDGFR and CSF1R, characterized by immunoglobulin-like domains and a split tyrosine kinase domain.
• Subcellular localization includes the cell membrane and cytoplasm; rapid internalization and degradation upon activation serve to modulate signaling intensity and duration.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation; alternative splicing generates multiple isoforms with distinct tissue distributions.
• Isoforms 1 and 2 are detected in bone marrow cells, spermatogonia, and spermatocytes; isoform 3 is found in round spermatids, elongating spermatids, spermatozoa, and bone marrow hematopoietic stem cells.
• Widely expressed in fetal liver and bone marrow, with isoform-specific expression in germ cells and hematopoietic stem cells.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning the extracellular domain (aa 25-523) of mouse CD117/c-Kit, which encompasses the ligand-binding region; the antibody may efficiently recognize native receptor on live cells.
• Validated for flow cytometry; suitable for analyzing CD117/c-Kit expression on mouse hematopoietic stem cells, mast cells, germ cells, or other Kit-expressing populations.
• For optimal flow cytometry results, consider using appropriate isotype controls, Fc receptor blocking, and viability dyes to minimize non-specific binding.
• If extending to other applications (e.g., Western blot, IHC), users should validate performance in their specific experimental system, as the antibody is currently verified only for FC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop sourcing solutions for IVD antibody raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-CD117/c-Kit antibody is suitable for stem cell biology and hematopoiesis research applications. Through our validated antibody pair platforms, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, we streamline procurement for your diagnostic and research development needs.