Rabbit anti-Mouse CD112/Nectin-2 Monoclonal Antibody for Flow Cytometry - P32507

Core Product Specifications and Parameters

Biological Background: CD112/Nectin-2 Function and Localization

• Nectin-2, also known as poliovirus receptor-related protein 2 or herpesvirus entry mediator B (HveB), is a cell adhesion protein and modulator of T-cell signaling.
• It can act as a costimulator of T-cell function upon binding to CD226, stimulating T-cell proliferation and cytokine production (e.g., IL2, IL5, IL10, IL13, IFNG). Alternatively, it can coinhibit T-cell proliferation when binding to PVRIG.
• Encoded by the Nectin2 gene, the protein belongs to the nectin family and contains immunoglobulin domains.
• Subcellularly, nectin-2 is localized to the cell membrane (single-pass transmembrane protein).
• Post-translational modifications include glycosylation and phosphorylation; the protein also contains disulfide bonds.
• Tissue specificity: expressed in brain, spinal cord, spleen, kidney, heart, and liver.
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , making it suitable for direct flow cytometry analysis without the need for a secondary antibody.
• The immunogen corresponds to the extracellular region (aa 32-345) of mouse CD112/Nectin-2, suggesting recognition of the cell surface form.
• For flow cytometry, consider using appropriate isotype controls and titrating the antibody to determine optimal staining concentrations in your experimental system.
• Since nectin-2 is expressed in lymphoid tissues (e.g., spleen) and brain, validate staining in relevant mouse cell lines or primary cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibodies, bulk reagents, and rare target sourcing. This anti-CD112/Nectin-2 antibody supports immunology and cell adhesion research, and CamelBio can assist with procurement of complementary raw materials and technical consultation.