Rabbit anti-Mouse CD105/Endoglin mAb for Flow Cytometry - Q63961

Core Product Specifications and Parameters

Biological Background: CD105/Endoglin Function and Localization

• Endoglin (CD105) is a vascular endothelium glycoprotein essential for angiogenesis, regulating vascular endothelial cell migration and required for normal adult vasculature integrity. Related references: PMID:10625534, PMID:17540773, PMID:28530658
• It functions as a TGF-beta coreceptor, involved in TGF-beta/BMP signaling cascades leading to SMAD transcription factor activation, specifically mediating GDF2/BMP9 signaling via SMAD1 and modulating TGFB1 signaling via SMAD3. Related references: PMID:23300529
• At the subcellular level, endoglin is a single-pass type I membrane protein localized to the cell membrane.
• It is characterized by structural features including a signal peptide, extracellular domain with a ZP domain, a transmembrane helix, and a cytoplasmic tail; post-translational modifications include N-glycosylation, disulfide bond formation, and phosphorylation.
• Tissue expression is predominantly restricted to endothelial cells in all tissues except bone marrow; additionally detected in some stromal cells of intestine, stomach, heart, skeletal muscle, uterus, ovary, oviduct, testis, and thymus. Related references: PMID:8194490, PMID:10625534
• Endoglin plays a critical role during embryonic development, being required for normal extraembryonic angiogenesis and embryonic heart development. Related references: PMID:10625534
• It may also regulate endothelial cell shape changes in response to blood flow, driving vascular remodeling and morphology establishment. Related references: PMID:28530658

Experimental Guidance and Technical Tips

• The immunogen covers the ectodomain (aa 27-581) of mouse CD105, which may favor recognition of the native conformation.
• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Use appropriate isotype controls and include single-stain controls for compensation when using multiple fluorophores.
• Validate antibody specificity by including negative controls such as cells from CD105 knockout models or receptor-blocking peptides where feasible.
• Ensure compatibility of the fluorophore with your instrument’s laser and filter configuration; avoid prolonged exposure to light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive, one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with high-quality antibodies and ancillary reagents. This -conjugated anti-mouse CD105 antibody supports vascular biology and angiogenesis research, and we can assist with further needs such as validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom rare-target raw-material procurement. Let CamelBio bridge your concept-to-clinic journey with reliable supply solutions.