Rabbit anti-Mouse CD105/Endoglin mAb for FC - Q63961

Core Product Specifications and Parameters

Biological Background: CD105/Endoglin Function and Localization

• Endoglin (CD105) is a transmembrane glycoprotein that acts as a vascular endothelium marker, playing a critical role in angiogenesis and vascular development (PubMed:10625534, PubMed:17540773).
  Related references: PMID:10625534 PMID:17540773

• It functions as a TGF-beta coreceptor, mediating BMP9/GDF2 signaling via SMAD1 and modulating TGFB1 signaling through SMAD3, thus influencing cell proliferation and migration (PubMed:23300529).
  Related references: PMID:23300529

• Regulates endothelial cell shape changes in response to blood flow, contributing to vascular remodeling and morphogenesis (PubMed:28530658).
  Related references: PMID:28530658

• Localized to the cell membrane, endoglin is expressed predominantly on endothelial cells but also found in stromal cells of various tissues including bone marrow, intestine, and heart (PubMed:8194490, PubMed:10625534).
  Related references: PMID:8194490 PMID:10625534

• The protein contains disulfide bonds, is glycosylated, and undergoes phosphorylation, contributing to its structural stability and signaling functions.

• Knockout studies in mice demonstrate its essential role in extraembryonic angiogenesis and embryonic heart development, highlighting its importance in cardiovascular biology (PubMed:10625534).
  Related references: PMID:10625534

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 27-581) of mouse CD105, making this antibody suitable for detecting cell surface expression by flow cytometry.
• For FC applications, consider titrating the antibody to optimize signal-to-noise ratio in your specific cell type and experimental conditions.
• Include appropriate isotype controls and fluorescence compensation controls to ensure specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, serving as a one-stop partner for diagnostic manufacturers and research institutes. This anti-CD105 antibody supports studies in angiogenesis, TGF-beta signaling, and vascular biology. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal options, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.