Rabbit anti-Mouse CD103 Monoclonal Antibody for Flow Cytometry - Q60677

Core Product Specifications and Parameters

Biological Background: Integrin alpha-E (CD103) Function and Localization

• Integrin alpha-E (CD103), also known as integrin alpha M290, is encoded by the Itgae gene in mouse and is a member of the integrin alpha chain family.
• It pairs non-covalently with integrin beta-7 (ITGB7) to form the alpha-E/beta-7 heterodimer, which serves as a receptor for epithelial cadherin (E-cadherin).
• The integrin alpha-E/beta-7 complex mediates the adhesion of intra-epithelial T lymphocytes to epithelial cell monolayers, facilitating tissue residency.
• CD103 is a single-pass type I membrane protein with a large extracellular domain containing ligand-binding and calcium-binding regions, a transmembrane helix, and a short cytoplasmic tail.
• The extracellular region includes several integrin alpha repeats and a von Willebrand factor type A domain (predicted), and the protein is N-glycosylated.
• As a cell adhesion molecule, CD103 plays a key role in mucosal immunity by retaining lymphocytes at epithelial sites.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region of mouse CD103 (aa 191-385), which includes part of the beta-propeller domain involved in ligand interaction; this region is conserved and suitable for functional studies.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type. Use known positive cells (e.g., intraepithelial T cells) and appropriate isotype controls.
• CD103 is expressed on the surface of intraepithelial T lymphocytes; confirm expression in your cellular system before use. Permeabilization is not required for surface staining.
• When using -conjugated antibodies in multicolor panels, ensure proper compensation and verify spectral compatibility with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop procurement platform for IVD raw materials, offering a broad portfolio of validated antibodies, antigens, and ancillary reagents. For mucosal immunology research, we provide monoclonal and polyclonal antibodies targeting CD103 and other integrins, along with optimized antibody pairs and bulk custom sourcing. Our team supports diagnostic manufacturers from feasibility to commercialization with reliable supply and technical guidance.