Rabbit anti-Mouse CD102 Monoclonal Antibody for Flow Cytometry - P35330

Core Product Specifications and Parameters

Biological Background: CD102 Function and Localization

• Intercellular adhesion molecule 2 (ICAM-2), also designated CD102 or Ly-60, is a 31kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily.
• Acts as a cell adhesion receptor ligand for the integrin ITGAL:ITGB2 (LFA-1), mediating leukocyte cell-cell adhesion on resting cells. Related references: PMID:1401904
• Serves as an endothelial receptor ligand for dendritic cell CD209 (DC-SIGN), facilitating transendothelial migration of dendritic cell precursors from blood into peripheral tissues.
• Participates in adhesive interactions critical for antigen presentation, lymphocyte recirculation, and immune surveillance.
• The protein is predicted to contain disulfide bonds and N-glycosylation sites, contributing to structural stability and ligand recognition.
• Localizes predominantly to the cell membrane and is enriched on microvillus projections of leukocytes and endothelial cells.
• Expressed at high levels in lung endothelium and on circulating leukocytes, consistent with its role in immune cell trafficking.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20-222), which includes the ligand-binding Ig-like repeats; this design may favor recognition of native CD102 on live cells, making it suitable for flow cytometry.
• The direct conjugation enables one-step staining; consider titrating the antibody to determine the optimal concentration for your cell type and experimental setup.
• For flow cytometry, validate reactivity using mouse endothelial cells or leukocytes known to express CD102, and include appropriate isotype controls and viability dyes.
• Although the calculated unglycosylated mass is 31kDa, glycosylation may cause a higher apparent molecular weight in denaturing gels; this does not impact detection by flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents, supporting diagnostic and research applications from concept to clinic. This anti-CD102 antibody targets a key molecule in immune cell adhesion and trafficking, relevant for immunology and inflammation studies. For your raw material needs—whether routine or rare—CamelBio is your trusted sourcing partner.