Rabbit anti-Mouse CD102 Monoclonal Antibody for Flow Cytometry - P35330

Core Product Specifications and Parameters

Biological Background: CD102 (Icam-2) Function and Localization

• CD102, also known as Intercellular adhesion molecule 2 (Icam-2) or Lymphocyte function-associated AG-1 counter-receptor, is a cell adhesion molecule that serves as a receptor ligand for the signaling receptor ITGAL:ITGB2 (LFA-1), ensuring leukocyte cell‑cell adhesion on resting cells.
  Related references:
  PMID:1401904

• The protein mediates adhesive interactions that are critical for antigen presentation, lymphocyte recirculation, and immune surveillance.

• Through interaction with dendritic cell receptor CD209, CD102 triggers transendothelial migration of dendritic cell precursors from blood into peripheral and lymphoid tissues.

• CD102 is localized at the cell membrane and is enriched on microvilli, a cell projection that facilitates initial leukocyte‑endothelial contacts.

• Tissue expression analysis shows constitutive presence on endothelial cells and leukocytes, with particularly high levels detected in lung tissue.

• Structural features include an immunoglobulin‑like domain, a disulfide bond, and N‑glycosylation, classifying CD102 as a member of the immunoglobulin superfamily of adhesion molecules.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20‑222) of mouse CD102, which includes the LFA‑1 binding region; this design supports recognition of the native conformation on live cells, making it suitable for flow cytometry applications.
• For optimal flow cytometry staining, consider titrating the conjugate to determine the saturating concentration in the relevant cell system, and include appropriate isotype and fluorescence minus one controls.
• Given the detection of CD102 on endothelial cells, validate the antibody in the specific tissue or cell type of interest, using known positive samples (e.g., mouse lung leukocytes) as benchmarks.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one‑stop sourcing partner for IVD raw materials, supplying high‑quality antibodies and reagents that empower diagnostic developers and researchers. This anti‑mouse CD102 monoclonal antibody exemplifies our ready‑to‑use conjugated solutions for immunology and cell adhesion research. Beyond individual products, CamelBio provides validated antibody pairs, bulk ancillary reagents, and expert sourcing for rare targets—streamlining your path from concept to clinical application.