Rabbit anti-Mouse CD10 Monoclonal Antibody for Flow Cytometry - Q61391

Core Product Specifications and Parameters

Biological Background: Neprilysin Function and Localization

• Neprilysin (CD10) is a zinc-dependent metalloprotease with thermolysin-like specificity, primarily cleaving polypeptides up to 30 amino acids (By similarity).
• It plays key roles in opioid peptide metabolism by degrading Met- and Leu-enkephalins via Gly-Phe bond cleavage (By similarity).
• Substrates include bradykinin, substance P, neurotensin, and angiotensins 1, 2, and 1-9, highlighting roles in cardiovascular and neuropeptide signaling (By similarity).
• It degrades atrial natriuretic factor (ANF), contributing to blood pressure and fluid balance regulation (By similarity).
• Neprilysin exhibits UV-inducible elastase activity on skin preelastic and elastic fibers, linking it to skin homeostasis (PubMed:20876573). Related references: PMID:20876573
• Localized to the cell membrane, it functions as a type II integral membrane protein.
• Key structural features include an N-terminal signal-anchor, a large extracellular catalytic domain, and multiple post-translational modifications such as N-glycosylation and phosphorylation, contributing to its stability and activity.

Experimental Guidance and Technical Tips

• As a directly conjugated antibody, it is ready for use in flow cytometry; titrate to determine the optimal staining concentration for your cell type.
• Include a matched isotype control (rabbit IgG, ) to assess nonspecific binding under identical experimental conditions.
• For intracellular staining, confirm that fixation and permeabilization buffers do not interfere with the fluorophore or epitope recognition.
• Validate reactivity in relevant mouse cell lines or primary cells known to express CD10, such as B cells, kidney, or skin tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories worldwide. This anti-mouse CD10 antibody exemplifies our ability to supply high-quality monoclonal antibodies for flow cytometry and other immunoassays, supported by bulk ordering and custom conjugation options. Whether your project focuses on neuropeptide signaling, cardiovascular biomarkers, or membrane protease research, our one-stop platform ensures streamlined procurement from early R&D to clinical application.