Rabbit anti-Mouse CD10 (Mme) Monoclonal Antibody for Flow Cytometry - Q61391

Core Product Specifications and Parameters

Biological Background: Neprilysin (CD10) Function and Localization

• Neprilysin (CD10, Mme) is a type II integral membrane zinc metalloprotease that cleaves peptide bonds on the amino side of hydrophobic residues, primarily targeting small peptides of up to 30 amino acids.
• It plays a critical role in the inactivation of neuropeptides and peptide hormones, including enkephalins, substance P, bradykinin, neurotensin, and angiotensins.
• CD10 degrades the atrial natriuretic factor (ANF), contributing to blood pressure regulation and fluid homeostasis.
• Beyond its endopeptidase function, CD10 exhibits UV-inducible elastase activity, breaking down skin preelastic and elastic fibers. Related references: PMID:20876573
• Subcellularly, neprilysin is anchored to the cell membrane via a single transmembrane domain, with its large extracellular domain containing the catalytic site.
• It is widely used as a diagnostic cell surface marker (CD10) in hematological and solid tissue malignancies, reflecting its expression on early lymphoid progenitors, germinal center B cells, and certain epithelial cells.
• The protein contains several functionally important post-translational modifications, including N-linked glycosylation and myristoylation, and is stabilized by multiple disulfide bonds.

Experimental Guidance and Technical Tips

• Since the antibody is directly conjugated to , it is ready-to-use for flow cytometry without secondary detection steps.
• The immunogen spans the majority of the extracellular domain (aa 52-750), ensuring recognition of the native membrane-bound form of CD10.
• Validate the antibody in the target mouse tissue or cell line of interest, considering CD10 expression patterns.
• For FC staining, titrate the antibody concentration to optimize signal-to-noise ratio in your specific sample system.
• Use appropriate isotype controls to assess non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to premium IVD raw materials like this anti-mouse CD10 monoclonal antibody, which is ideal for developing flow cytometry-based assays in immunophenotyping and cancer research. Beyond single antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your diagnostic development from concept to clinic.