Rabbit anti-Mouse CD10 mAb for FC - Q61391

Core Product Specifications and Parameters

Biological Background: CD10 (Neprilysin) Function and Localization

• CD10, also known as neprilysin, atriopeptidase, enkephalinase, neutral endopeptidase 24.11 (NEP), or skin fibroblast elastase (SFE), is a membrane-bound zinc metalloprotease encoded by the Mme gene.
• It exhibits thermolysin-like specificity and preferentially cleaves polypeptides of up to 30 amino acids, playing a key role in the inactivation of diverse signaling peptides.
• Neprilysin is critically involved in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleaving the Gly-Phe bond, thereby modulating pain and analgesic pathways.
• It cleaves vasoactive peptides including bradykinin, substance P, and neurotensin, contributing to the regulation of inflammation and vascular tone.
• The enzyme can process the angiotensin peptide cascade, cleaving angiotensin-1, angiotensin-2, and angiotensin 1-9, implicating it in blood pressure control.
• Neprilysin degrades atrial natriuretic factor (ANF), a cardiac hormone involved in sodium homeostasis and cardiovascular function.
• Under UV exposure, neprilysin displays elastase activity toward skin preelastic and elastic fibers, suggesting a role in photoaging and extracellular matrix remodeling. Related references: PMID:20876573
• The protein localizes to the cell membrane as a type II integral membrane protein, anchoring its active site to the extracellular face for efficient peptide hydrolysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein covering amino acids 52-750 of mouse CD10, encompassing the large extracellular catalytic domain. This design likely ensures recognition of native and denatured forms of the target.
• As a rabbit monoclonal antibody directly conjugated to , it is optimized for flow cytometry with minimal background. Titration experiments (e.g., 0.1–1 µg per million cells) are recommended to determine the optimal working concentration for your specific cell type and staining conditions.
• Include appropriate controls in flow cytometry assays: unstained cells, isotype control conjugated to the same fluorophore, and fluorescence-minus-one (FMO) controls if using multicolor panels.
• Since CD10 is a cell surface antigen, live-cell staining without permeabilization is typically sufficient for most applications. However, if intracellular staining is required, validate the compatibility of fixation/permeabilization buffers with the antibody’s reactivity.
• For best performance, protect the antibody from prolonged light exposure to maintain fluorophore stability and avoid freeze/thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop platform, CamelBio supplies diagnostic manufacturers, core labs, and research institutes with a comprehensive range of IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and hard-to-find targets. This anti-CD10 antibody supports critical investigations into neuropeptide regulation, cardiovascular physiology, and extracellular matrix biology. Whether you need off-the-shelf catalog products or custom raw-material sourcing, our team accelerates your transition from concept to clinic with responsive technical support and reliable supply chains.