Rabbit anti-Megalin/LRP2 Monoclonal Antibody for IF-P and FC (intra) - P98164

Core Product Specifications and Parameters

Biological Background: Megalin/LRP2 Function and Localization

• Megalin (LRP2) is a type I transmembrane glycoprotein of ~521 kDa, belonging to the low-density lipoprotein receptor family, characterized by multiple ligand-binding repeats, EGF-like domains, and a short cytoplasmic tail.
• It functions as a multiligand endocytic receptor, mediating the internalization and lysosomal degradation of diverse ligands, including lipoproteins, vitamin-carrier complexes, hormones, enzymes, and drugs, often in partnership with cubilin (CUBN).
• In the kidney, megalin is critical for tubular reabsorption of filtered proteins; it mediates uptake of metallothionein-bound heavy metals (e.g., cadmium, mercury), the anti-apoptotic protein survivin/BIRC5, vitamin D3/GC-DBP complexes, myoglobin, and matrix metalloproteinases, thereby preserving kidney function. Related references: PMID:15126248, PMID:23825075
• In the central nervous system, megalin transports leptin across the blood-brain barrier, participates in sonic hedgehog (SHH) clearance and transcytosis, and facilitates folate uptake essential for neural tube closure; it also mediates beta-amyloid clearance and supports neurogenesis by modulating BMP signaling.
• Megalin is localized primarily to the apical plasma membrane of polarized absorptive epithelia, such as renal proximal tubule cells, and undergoes clathrin-mediated endocytosis into endosomes.
• Tissue expression is prominent in the placenta (first and third trimester cytotrophoblasts) and renal proximal tubules, as confirmed by protein-level analyses. Related references: PMID:27798286
• Additional physiological roles include reproductive tissue uptake of sex hormone-binding globulin (SHBG)-bound androgens/estrogens, auditory function maintenance, and embryonic heart development.
• The protein is subject to calcium binding, phosphorylation, and extensive glycosylation, and mutations in LRP2 are associated with Donnai-Barrow syndrome and deafness.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal cytoplasmic region (aa 4446–4655), which may allow detection of full-length and certain processed forms of LRP2 in denatured and native states.
• For immunofluorescence on paraffin-embedded tissues (IF-P), consider a heat-induced antigen retrieval step with a pH 6.0 citrate buffer for optimal epitope exposure.
• For intracellular flow cytometry (FC), fixation and permeabilization are mandatory; pilot titrations (e.g., 0.5–5 µg/mL) in the relevant cell type are recommended to determine the optimal staining concentration.
• Although validated for IF-P and FC, this monoclonal antibody may perform in other applications; independent verification is advised, especially given the large size and complex structure of megalin.
• Cross-reactivity with mouse and rat megalin suggests conserved epitope sequences; however, users should confirm reactivity in their specific tissue or cell system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, supporting every stage from concept to clinic. For renal pathology, neuroscience, and drug uptake research, we supply validated antibodies like this anti-Megalin/LRP2 monoclonal antibody, along with complementary antibody pairs, recombinant antigens, and bulk ancillary reagents. Our team also specializes in rare target raw-material procurement to meet your specific assay development needs.