Rabbit anti-Human/Mouse/Monkey FOXP3 Monoclonal Antibody for Intracellular Flow Cytometry - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 is a forkhead box transcription factor essential for the development and suppressive function of regulatory T-cells (Treg), maintaining immune homeostasis. Related references: PMID:17377532, PMID:21458306, PMID:23947341, PMID:23169781
• It can act as either a transcriptional repressor or activator, depending on interactions with cofactors such as histone acetylases and deacetylases. Related references: PMID:17377532, PMID:21458306, PMID:23947341
• FOXP3 upregulates Treg signature genes (e.g., CTLA4, TNFRSF18) and represses pro-inflammatory cytokines including IL2 and IFNG. Related references: PMID:17377532, PMID:21458306, PMID:23947341
• It directly inhibits the transcription factors RELA and NFATC2 to suppress T-cell effector cytokine production. Related references: PMID:15790681
• FOXP3 mediates IL2 repression through association with histone acetylase KAT5 and histone deacetylase HDAC7. Related references: PMID:17360565
• Interaction with RUNX1 orchestrates the activation of CTLA4 and IL2RA while repressing IL2 and IFNG. Related references: PMID:17377532
• FOXP3 antagonizes RORC to hinder Th17 differentiation, thereby favoring Treg lineage commitment. Related references: PMID:18368049
• It inhibits the transcriptional activity of RORA. Related references: PMID:18354202
• Located in both the nucleus and cytoplasm; its activity is modulated by post-translational modifications such as acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The antibody is conjugated to , an Alexa Fluor® 647-equivalent dye, and is intended for intracellular flow cytometry. For optimal nuclear FOXP3 detection, fixation and permeabilization protocols suitable for transcription factors (e.g., using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set or similar) are recommended.
• The immunogen corresponds to an N-terminal peptide (amino acids 1–100) of human FOXP3. Verify epitope accessibility and specificity in your experimental system, as the antibody recognizes human, mouse, and cynomolgus FOXP3.
• When designing multicolor panels, consider the spectral properties of and include appropriate compensation controls.
• If adapting this antibody for other applications (e.g., Western blot), validate the observed band at the expected molecular weight of ~47 kDa, noting that post-translational modifications may cause shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, from early-stage research to clinical application. This anti-FOXP3 monoclonal antibody supports immunology and autoimmune disease investigations with reliable Treg detection. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and custom rare-target sourcing—streamlining your development workflow.