Rabbit anti-Human/Mouse/Monkey FOXP3 Monoclonal Antibody for Flow Cytometry - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 is a forkhead box transcription factor that serves as a master regulator of regulatory T cell (Treg) development and suppressive function, crucial for immune homeostasis. Related references: PMID:17377532, PMID:21458306, PMID:23947341, PMID:23169781
• It functions as a context-dependent transcriptional activator or repressor, modulating key immune genes such as upregulating CTLA4 and TNFRSF18 while repressing IL2 and IFNG. Related references: PMID:17377532, PMID:21458306, PMID:23947341, PMID:17360565
• FOXP3 inhibits effector T-cell cytokine production by repressing transcription factors NFATC2 and RELA. Related references: PMID:15790681
• It suppresses Th17 cell differentiation by antagonizing the transcription factor RORC, thereby promoting Treg lineage stability. Related references: PMID:18368049, PMID:18354202
• The protein localizes to both the nucleus and cytoplasm, consistent with its transcriptional and regulatory roles.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may regulate its stability and activity.
• Associated with immune dysregulation diseases such as IPEX syndrome (Immune dysregulation, Polyendocrinopathy, Enteropathy, X-linked syndrome), as indicated by its alias and disease variant keyword.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, optimize fixation and permeabilization protocols; since FOXP3 is a nuclear protein, use buffers designed for nuclear antigens to ensure epitope accessibility.
• The immunogen covers the N-terminal region (aa 1–100); validate the antibody in relevant sample systems and consider potential reactivity with isoforms or cleavage products.
• Titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions, using appropriate Fc blocking and isotype controls to minimize non-specific binding.
• provides bright and photostable fluorescence; avoid prolonged light exposure and include fluorescence minus one (FMO) controls to accurately set gates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and researchers with comprehensive sourcing solutions for critical raw materials. This FOXP3 antibody exemplifies our capability to supply validated monoclonal antibodies for immune-oncology and autoimmune disease research. From optimized antibody pairs to bulk ancillary reagents, CamelBio supports your workflow from concept to clinic.