Rabbit anti-Human/Mouse PDGFRα/CD140a Monoclonal Antibody for FC - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRα Function and Localization

• Platelet-derived growth factor receptor alpha (PDGFRα), also designated CD140a or PDGFR2, is a cell-surface tyrosine kinase receptor encoded by the PDGFRA gene.
• It serves as a receptor for PDGFA, PDGFB, and PDGFC, playing essential roles in embryonic development, cell proliferation, survival, chemotaxis, and mesenchymal stem cell differentiation.
• PDGFRα can form heterodimers with PDGFRβ, modulating downstream signaling outputs depending on the ligand context.
• Subcellularly, it localizes to the cell membrane, cell projection cilium, and Golgi apparatus.
• The protein is widely expressed, including in platelets, brain, fibroblasts, smooth muscle, heart, and embryonic tissues; it is also detected in normal colon and colon tumors.
• Post-translational modifications include disulfide bond formation, glycosylation, phosphorylation, and ubiquitin conjugation; it participates in key pathways such as PI3K/AKT, MAPK/ERK, and STAT.
• PDGFRα contributes to wound healing by promoting cell migration and is involved in platelet activation and thrombin-induced aggregation.
• Keywords indicate its role as a proto-oncogene and association with disease variants and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24–524), making this antibody suitable for surface staining of live cells in flow cytometry.
• For FC, titrate the antibody to determine the optimal working dilution for your specific cell type and experimental conditions.
• Use appropriate isotype controls and include Fc receptor blocking steps to minimize non-specific binding.
• The conjugate is compatible with the 488 nm laser line; ensure adequate compensation when used in multicolor panels.
• Reported cross-reactivity with human and mouse; when working with other species, verify reactivity empirically due to potential sequence homology.
• Consider pre-incubating cells with recombinant PDGFRα protein to confirm specificity in competitive binding assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing reliable access to IVD raw materials like this PDGFRα/CD140a antibody. Backed by validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, we facilitate your development of PDGF signaling-related assays. Our sourcing capabilities extend to rare targets and custom specifications, bridging your concept-to-clinic needs.