Rabbit anti-Human/Mouse PDGFRα (CD140a) Monoclonal Antibody for FC - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRα Function and Localization

• PDGFRα (platelet-derived growth factor receptor alpha) is a cell-surface tyrosine kinase receptor essential for embryonic development, cell proliferation, survival, and chemotaxis. It binds PDGFA, PDGFB, and PDGFC ligands.
• The receptor forms homodimers or heterodimers with PDGFRβ, activating multiple signaling cascades including PI3K/AKT, RAS/MAPK, and STAT pathways.
• PDGFRα is widely expressed, notably in brain, fibroblasts, smooth muscle, heart, and platelets; it is also detected in colon tumors and normal colon tissue.
• Subcellularly, PDGFRα localizes to the cell membrane, cilium, and Golgi apparatus.
• Key post-translational modifications include phosphorylation (its activation hallmark), N-linked glycosylation, and ubiquitination, which regulate signaling and turnover.
• Alternatively spliced isoforms and proteolytic processing yield protein species of molecular weights approximately 24, 82, and 122 kDa (full-length).
• PDGFRα plays critical roles in skeleton development, gastrointestinal mucosal formation, wound healing, and platelet activation.
• As a proto-oncogene, mutations and overexpression in PDGFRA are implicated in cancers such as gastrointestinal stromal tumors (GISTs) and glioblastoma.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 24-524), suggesting the antibody likely recognizes the native receptor conformation on live cells, suitable for flow cytometry.
• With reported cross-reactivity for both human and mouse, this antibody can be used in murine models; validation in the relevant sample system is recommended.
• The target protein has multiple molecular weight forms (24, 82, 122 kDa) due to alternative splicing and post-translational modifications; when adapting for western blot, these bands should be considered and confirmed with appropriate controls.
• For flow cytometry, titrate the antibody to determine optimal concentration and include isotype controls to ensure specificity.
• Note that PDGFRα internalization upon ligand binding may affect staining; consider ligand-free conditions if assessing surface expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with a comprehensive one-stop sourcing platform for IVD raw materials, from development to clinic. This PDGFRα (CD140a) monoclonal antibody serves oncology and developmental biology research, and we offer extended supply capabilities including validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target sourcing to support your assay scale-up and commercial needs.