Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) Monoclonal Antibody for Flow Cytometry - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (ITGA5), also known as CD49e, VLA-5, or fibronectin receptor subunit alpha, pairs with integrin beta-1 (ITGB1) to form the major fibronectin receptor, mediating cell-extracellular matrix adhesion.
• ITGA5:ITGB1 recognizes the R-G-D sequence in fibronectin and fibrinogen, serving as a classical ligand-binding receptor.
• ITGA5:ITGB1 interacts with PLA2G2A via a non-classical site (site 2), inducing conformational changes that enhance ligand binding to site 1. Related references: PMID:18635536, PMID:25398877
• It acts as a receptor for fibrillin-1 (FBN1) and fibronectin (FN1), mediating R-G-D-dependent cell adhesion to these matrix proteins. Related references: PMID:12807887, PMID:17158881, PMID:33962943
• ITGA5:ITGB1 functions as a receptor for IL1B, essential for IL1B signaling, and for soluble CD40LG, required for CD40/CD40LG signaling. Related references: PMID:29030430, PMID:31331973
• In microbial infections, ITGA5:ITGB1 acts as a receptor for Human metapneumovirus; ITGA2:ITGB1 acts as a receptor for Human parvovirus B19; and interaction with HIV-1 Tat protein enhances angiogenesis in Kaposi's sarcoma lesions.
• Subcellular localization includes the cell membrane and focal adhesions. ITGA5 is a glycoprotein with calcium-binding, metal-binding, and phosphorylation sites; it is expressed in placenta at the protein level.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering human and mouse ITGA5, suggesting potential cross-species suitability; always validate in the relevant sample system.
• For flow cytometry, consider using appropriate isotype controls and staining buffers optimized for integrin family proteins, which may require divalent cations (Ca²⁺/Mg²⁺) for proper conformation.
• Integrin expression can vary by cell type and activation state; include positive and negative cellular controls to confirm staining specificity.

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