Rabbit anti-Human/Mouse FOXP3 Monoclonal Antibody for FC (intra) - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 (Forkhead box protein P3, also known as Scurfin) is a forkhead transcription factor critical for immune regulation, with multiple isoforms arising from alternative splicing (predicted molecular weights ~44–50 kDa).
• It is essential for the development and suppressive function of regulatory T-cells, maintaining immune homeostasis by stabilizing the Treg lineage and modulating conventional T-cell expansion. Related references: PMID:17377532 PMID:21458306 PMID:23947341 PMID:23169781
• FOXP3 acts as both a transcriptional repressor and activator, with its activity modulated by interactions with other transcription factors, histone acetylases, and deacetylases. Related references: PMID:17377532 PMID:21458306 PMID:23947341
• The suppressive activity of Treg involves FOXP3-coordinated activation of genes like CTLA4 and TNFRSF18, and repression of cytokines IL-2 and IFN-γ; it also interacts with RUNX1 to modulate these targets. Related references: PMID:17377532 PMID:21458306 PMID:23947341
• FOXP3 represses IL-2 transcription by recruiting the histone acetylase KAT5 and histone deacetylase HDAC7. Related references: PMID:17360565
• It inhibits Th17 cell differentiation by antagonizing the transcription factor RORC, thereby down-regulating IL-17 expression. Related references: PMID:18368049
• FOXP3 localizes predominantly in the nucleus but can also be detected in the cytoplasm, and its activity is regulated by post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of FOXP3, a sequence highly conserved between human and mouse, supporting the antibody's cross-reactivity.
• For intracellular flow cytometry, use optimized fixation and permeabilization protocols, and consider co-staining with surface Treg markers (e.g., CD4, CD25) for accurate gating.
• Validate the antibody in your specific model system using appropriate positive (e.g., Treg cells) and negative (e.g., conventional T-cells) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for sourcing high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents tailored for Treg biology and immune regulation research. We provide end-to-end support from early development to clinical applications, ensuring reliable supply chain solutions for diagnostic manufacturers and research laboratories. Reach out to explore bulk ordering and customized formulation options for your FOXP3-related projects.