ABflo 647 Rabbit anti-Human/Mouse CD276/B7-H3 Monoclonal Antibody for Flow Cytometry - Q5ZPR3-2

Core Product Specifications and Parameters

Biological Background: CD276 (B7-H3) Function and Localization

• CD276, designated as B7 homolog 3 (B7-H3), is a costimulatory molecule belonging to the B7 immunoglobulin superfamily.
• Isoform 2 (UniProt: Q5ZPR3-2) is a splice variant with a calculated molecular weight of approximately 33.8 kDa; multiple forms may exist due to alternative splicing.
• The protein's extracellular region, targeted by this antibody (amino acids 29-245), mediates interactions with putative receptors on immune cells.
• Alternative aliases include 4Ig-B7-H3 and B7RP-2, reflecting structural diversity.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein covering amino acids 29-245, which corresponds to the extracellular domain; this design likely ensures recognition of the native conformation in flow cytometry.
• Since this antibody is conjugated to ABflo 647, it is ready-to-use for direct immunofluorescence staining; consider optimizing the dilution for each experimental system.
• Validate the antibody's performance in your specific sample type (human or mouse) using appropriate controls, given the cross-reactivity indicated.
• The antibody may detect multiple bands in Western blotting (if used) due to isoforms; flow cytometry applications typically do not require such validation, but fixation/permeabilization conditions should be tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including monoclonal antibodies like this CD276/B7-H3 clone. Our portfolio spans validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us for reliable supply solutions supporting your immuno-oncology research and assay development.