Rabbit anti-Human/Mouse CD276/B7-H3 Monoclonal Antibody for Flow Cytometry - Q5ZPR3-2

Core Product Specifications and Parameters

Biological Background: CD276/B7-H3 Function and Localization

• CD276/B7-H3 is a member of the B7 family of immune regulatory proteins, functioning as a costimulatory molecule.
• It is also known by aliases 4Ig-B7-H3, B7 homolog 3 (B7-H3), and B7RP-2.
• The protein plays a role in modulating T-cell responses and is implicated in both immune activation and suppression.
• The gene encoding CD276 is designated CD276 (also B7H3) and is located on human chromosome 15.
• Isoform 2 (UniProt: Q5ZPR3-2) is a specific variant with a calculated molecular weight of 33791 Da.

Experimental Guidance and Technical Tips

• The antibody was raised against amino acids 29–245, corresponding to the extracellular domain of CD276/B7-H3. This region is likely accessible on the cell surface, making it suitable for flow cytometry.
• As an conjugate, it is ready-to-use for direct immunofluorescence. Protect from light and avoid repeated freeze-thaw cycles to maintain fluorophore stability.
• For flow cytometry, titrate the antibody to determine the optimal working dilution for your specific cell type and experimental conditions. Include appropriate isotype controls.
• Cross-reactivity with both human and mouse CD276 has been reported, enabling preclinical studies in mouse models. Validate performance in your target tissue or cell line.
• Consider using this antibody for immune checkpoint research, particularly in oncology and autoimmune disease contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and tailored procurement solutions. This anti-CD276/B7-H3 monoclonal antibody supports immune checkpoint research, and CamelBio can facilitate access to related reagents, including recombinant proteins, conjugated antibodies, and rare target raw materials, to streamline your assay development from concept to clinic.