Rabbit anti-Human/Monkey Ki67 Monoclonal Antibody for Intracellular Flow Cytometry - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Proliferation marker protein Ki-67 (antigen KI-67, MKI67) is a large nuclear protein (358.7 kDa) that associates with the surface of mitotic chromosomes and plays dual roles as a chromosome repellent during early mitosis and an attractant during late mitosis.
• In early mitosis, Ki-67 forms extended brush structures on chromosomes, preventing their collapse into a single chromatin mass via steric and electrostatic charge barriers, thus enabling independent chromosome motility. Related references: PMID:27362226
• During mitotic anaphase, dephosphorylation of Ki-67 and exposure of a conserved basic patch induce RNA-dependent liquid-liquid phase separation on the chromosome surface, switching it to a chromosome attractant to promote clustering and exclusion of cytoplasmic particles from the future nuclear space. Related references: PMID:32879492, PMID:39153474
• Ki-67 binds DNA, with a preference for supercoiled and AT-rich DNA, and also binds premature ribosomal RNAs during anaphase, contributing to phase separation. Related references: PMID:10878551, PMID:28935370
• The protein localizes to chromosomes, the nucleus, and prominently the nucleolus during interphase.
• It undergoes multiple post-translational modifications including phosphorylation, acetylation, and isopeptide bond formation, and is subject to alternative splicing and ubiquitination (keywords: Acetylation, Alternative splicing, Phosphoprotein, Ubl conjugation).
• Alternative splicing generates multiple isoforms of MKI67, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 2271–2370 of human Ki67, ensuring epitope specificity for intracellular detection.
• For intracellular flow cytometry, thoroughly optimize fixation and permeabilization conditions to allow antibody access to nuclear Ki-67 while preserving epitope integrity.
• Consider titrating the conjugate to determine the optimal concentration for your cell type and staining protocol.
• Validate the antibody in proliferating human and monkey cells, using appropriate isotype controls and unstimulated or G0-arrested cells as negative controls.
• Avoid prolonged light exposure to protect the fluorophore; follow standard practices for conjugated antibody storage and handling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials. This Ki-67 antibody exemplifies our commitment to supporting cell proliferation and oncology research with high-quality monoclonal antibodies. Whether you need validated antibody pairs for immunoassays, bulk ancillary reagents, or specialized raw-material sourcing, CamelBio acts as your single-window procurement partner.