Rabbit anti-Human/Monkey Ki67 Monoclonal Antibody for Intracellular Flow Cytometry - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Proliferation marker protein Ki-67, also known as antigen KI-67, encoded by the MKI67 gene. Multiple isoforms exist due to alternative splicing.
• Core mitotic chromosome function: Associates with chromosome surface during mitosis, acting as a chromosome repellent in early mitosis and an attractant in late mitosis to maintain chromosome individualization and facilitate nuclear reformation. Related references: PMID:27362226 PMID:32879492 PMID:35513709
• Forms extended brush-like structures on chromosomes, creating steric and electrostatic barriers that prevent chromosome collapse; its high net charge acts as a surfactant. Related references: PMID:27362226
• During anaphase, the brush collapses and Ki-67 promotes chromosome clustering, aiding in exclusion of large cytoplasmic particles from the future nucleus. Related references: PMID:32879492 PMID:39153474
• Dephosphorylation during mitotic exit exposes a conserved basic patch, inducing RNA-dependent liquid-like condensation on chromosomes that promotes coalescence of chromosome surfaces. Related references: PMID:39153474 PMID:28935370
• Binds DNA with a preference for supercoiled and AT-rich DNA. Related references: PMID:10878551
• Subcellular localization and PTMs: Localizes to chromosomes, nucleus, and nucleolus; undergoes phosphorylation, acetylation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen region corresponds to the C-terminal portion of Ki67; for intracellular flow cytometry, ensure proper fixation and permeabilization conditions for nuclear antigen exposure.
• As a direct conjugate, this antibody enables one-step staining, minimizing non-specific binding commonly seen with secondary antibodies.
• Ki67 is a well-established marker for proliferating cells; consider co-staining with DNA content dyes for cell cycle phase analysis.
• Cross-reactivity with cynomolgus monkey allows for translational studies; validate staining patterns in your specific model using known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, offering validated antibody pairs, monoclonal antibodies, and bulk reagents for diagnostic development. This anti-Ki67 antibody fits seamlessly into cancer diagnostic and research panels, and our team can source rare targets, ancillary reagents, and provide technical consulting from concept to clinic.