Rabbit anti-Human/Monkey Ki67 Monoclonal Antibody for Flow Cytometry - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Ki67 is a nuclear protein widely used as a proliferation marker, with two isoforms of approximately 319 kDa and 359 kDa due to alternative splicing.
• During early mitosis, Ki67 relocalizes from nucleoli to the chromosome surface, forming a brush-like structure that prevents chromosome aggregation via steric and electrostatic repulsion, ensuring independent chromosome motility. Related references: PMID:27362226
• At anaphase, dephosphorylation triggers a functional switch: Ki67 collapses into a condensed phase that attracts chromosomes together, facilitating nuclear envelope reformation and exclusion of cytoplasmic particles. Related references: PMID:32879492 PMID:39153474
• Ki67 binds premature ribosomal RNAs during anaphase, promoting liquid-liquid phase separation on the chromosome surface, which is critical for chromosome clustering. Related references: PMID:28935370 PMID:39153474
• The protein also binds DNA with a preference for supercoiled and AT-rich sequences, though its direct role in chromatin organization remains unclear. Related references: PMID:10878551
• Subcellular localization: Ki67 is predominantly found in the nucleolus during interphase, associates with condensing chromosomes in mitosis, and coats the entire chromosome surface.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which regulate its mitotic functions and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 2271-2370 of human Ki67, located in the C-terminal region. Ensure that the target epitope is accessible under the intended fixation and permeabilization conditions.
• For intracellular flow cytometry (FC intra), use permeabilization buffers compatible with nuclear antigens. Include appropriate isotype controls and validate staining in a known proliferating cell line (e.g., HeLa, Jurkat) versus a non-proliferating control.
• Cross-reactivity with cynomolgus monkey is reported; verify in your specific experimental system if working with other species.
• The conjugated fluorophore is a substitute for Pacific Blue/eFluor 450; ensure instrument compatibility and avoid prolonged light exposure.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline your diagnostic development by providing a one-stop sourcing platform for IVD raw materials, including validated antibodies, recombinant proteins, and auxiliary reagents. This anti-Ki67 monoclonal antibody is part of our portfolio for cell proliferation and oncology research, backed by our capability to supply custom antibody pairs, bulk orders, and rare-target raw materials. Leverage our expertise to accelerate your assay development from concept to clinic.