Rabbit anti-Human/Monkey Ki67 Monoclonal Antibody for Flow Cytometry (Intracellular) - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Proliferation marker protein Ki-67, encoded by the MKI67 gene, also known as antigen KI-67, MIB-1, KIA, and PPP1R105.
• Acts as a mitotic chromosome organizer: functions as a chromosome repellent during early mitosis to maintain individual chromosome dispersion, and as a chromosome attractant during late mitosis to promote chromosome clustering (PubMed:27362226, PubMed:32879492, PubMed:35513709). Related references: PMID:27362226 PMID:32879492 PMID:35513709
• Forms an extended brush-like structure on the chromosome surface, providing a steric and electrostatic barrier that prevents chromosome collapse; during anaphase, dephosphorylation triggers RNA-dependent liquid-liquid phase separation to promote chromosome clustering (PubMed:27362226, PubMed:32879492, PubMed:39153474). Related references: PMID:27362226 PMID:32879492 PMID:39153474
• Binds DNA with preference for supercoiled and AT-rich DNA, and also binds premature ribosomal RNA during anaphase, facilitating phase separation (PubMed:10878551, PubMed:28935370, PubMed:39153474). Related references: PMID:10878551 PMID:28935370 PMID:39153474
• Subcellular location: resides in the nucleolus during interphase; relocalizes to the surface of mitotic chromosomes during mitosis.
• Subject to post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its function and cell cycle-dependent dynamics.
• Designated as a proliferation marker due to its cell cycle-dependent expression pattern.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (aa 2271-2370) of human Ki67. Confirm that the epitope is accessible in your intracellular staining protocol.
• This antibody is directly conjugated to for one-step intracellular flow cytometry. Optimize fixation and permeabilization conditions for your cell type to preserve the Ki67 epitope while ensuring antibody access to the nucleus.
• Cross-reactivity with cynomolgus monkey (Macaca fascicularis) suggests suitability for non-human primate studies; validate cross-reactivity in your specific model system.
• Ki67 is a large nuclear protein (predicted molecular weight ~359 kDa), so ensure adequate permeabilization and consider using a nuclear counterstain for gating.
• For quantitative proliferation analysis, combine with a DNA content dye (e.g., propidium iodide) in a bivariate flow cytometry assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-Ki67 antibody supports cell proliferation research in oncology and immunology, and our team can assist with integrating it into your diagnostic or research workflow. From concept to clinic, CamelBio delivers reliable raw materials and technical expertise to accelerate your IVD development.