Rabbit anti-Human/Monkey IgM Monoclonal Antibody for IF/ICC & FC - P01871-1

Core Product Specifications and Parameters

Biological Background: IgM Function and Localization

• Immunoglobulin heavy constant mu (IgM heavy chain) is the constant region of secreted IgM, encoded by the IGHM gene. It multimerizes to form high-order polymers, predominantly pentamers, enabling multivalent antigen binding (PubMed:32029689, PubMed:37095205). Related references: PMID:32029689 PMID:37095205
• Natural secreted IgM (sIgM) is polyreactive and recognizes conserved self- and pathogen-derived structures, whereas immune sIgM is antigen-specific and produced upon pathogen exposure; both are essential for effective humoral immunity.
• IgM mediates effector functions via Fc receptors (such as FCMR on lymphoid cells) and activates the classical complement pathway by binding C1q, promoting pathogen neutralization and clearance (PubMed:12847249, PubMed:19006321, PubMed:28230186). Related references: PMID:12847249 PMID:19006321 PMID:28230186
• It plays a critical role in mucosal immunity: sIgM is transported across mucosal epithelia via the polymeric immunoglobulin receptor (PIGR) and released as secretory IgM, where it scans for pathogens (PubMed:32029689). Related references: PMID:32029689
• IgM, together with C1q and mannose-binding lectin, facilitates macrophage phagocytosis of apoptotic cells, aiding in the clearance of potential autoimmune epitopes.
• The protein is secreted and has a molecular mass of approximately 49.4 kDa.
• Isoform 1 of the IgM constant region is also known as Ig mu chain C region variants BOT, GAL, and OU.

Experimental Guidance and Technical Tips

• This -conjugated rabbit monoclonal antibody targets amino acids 218-453 of the human IgM constant region (P01871-1). It has been validated for immunofluorescence/immunocytochemistry (IF/ICC) and flow cytometry (FC) applications.
• For IF/ICC, consider optimizing fixation and permeabilization conditions to preserve the IgM epitope, and use appropriate controls including isotype controls and secondary antibody-only controls.
• In flow cytometry, titrate the antibody to determine the optimal working dilution for your specific cell type; ensure single-cell suspensions and include Fc receptor blocking if needed.
• The antibody cross-reacts with Cynomolgus IgM, making it suitable for non-human primate studies; validate cross-reactivity in your target tissue if extending to other species.
• Since IgM is secreted, intracellular staining may require Golgi inhibitors to detect newly synthesized IgM; however, surface staining directly identifies membrane-bound IgM on B cells.
• For IVD assay development, verify antibody performance in your specific sample matrix (e.g., serum, plasma) and detection platform.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive range of IVD raw materials. This anti-IgM antibody, targeting a key immunoglobulin for humoral immunity, is part of our portfolio of validated monoclonal antibodies designed for immunology and infectious disease assay development. Beyond individual antibodies, we offer bulk reagent sourcing, customized technical support, and integrated solutions to accelerate your diagnostic projects from concept to clinic.