Rabbit anti-Human/Monkey IgM Monoclonal Antibody for Flow Cytometry - P01871

Core Product Specifications and Parameters

Biological Background: IgM Function and Localization

• IGHM encodes the constant region of immunoglobulin heavy chain mu, which defines the IgM isotype. IgM exists as both membrane-bound B cell receptor (BCR) and secreted antibody, playing central roles in humoral immunity. Related references: PMID:20176268, PMID:22158414

• The antigen binding site is formed by the variable domain of one heavy chain and one light chain, assembled through V-(D)-J rearrangement and subjected to somatic hypermutations for affinity maturation after antigen exposure. Related references: PMID:17576170, PMID:20176268

• Secreted IgM (sIgM) multimerizes predominantly into pentamers, enabling high-avidity antigen binding and efficient pathogen neutralization via complement activation (C1q recruitment) and Fc receptor-mediated effector functions. Related references: PMID:32029689, PMID:37095205, PMID:12847249, PMID:19006321, PMID:28230186

• Membrane-bound IgM is a component of the BCR complex, providing tonic signaling essential for B cell survival and mediating pre-BCR signaling for allelic exclusion.

• IgM is involved in mucosal immunity: it is transported across epithelia by PIGR and can undergo retrotranscytosis via FCMR to present antigens. Related references: PMID:32029689

• Subcellular localization includes secreted forms found in bodily fluids and membrane-bound forms on the surface of B lymphocytes.

• Key post-translational modifications include disulfide bonds that stabilize the multimeric structure and glycosylation that contributes to stability and effector functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 218–453 of the human IgM constant region (P01871), likely ensuring specificity for the mu heavy chain.
• For flow cytometry, consider titrating the conjugate to determine the optimal staining index in your cell system.
• Include appropriate isotype controls and viability dyes to distinguish specific surface IgM staining on live B cells.
• Validate performance in a relevant sample system, such as human PBMCs or Cynomolgus monkey lymphocytes, to confirm cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with efficient access to validated raw materials like the Rabbit anti-IgM mAb, which targets a cornerstone of humoral immunity. As a comprehensive sourcing partner, we can supply this antibody in bulk, along with optimized antibody pairs and ancillary reagents, to support your immunoassay development pipeline. We also assist in sourcing rare-target raw materials to meet specific IVD needs.