Rabbit anti-Human/Monkey IgM Monoclonal Antibody for FC - P01871

Core Product Specifications and Parameters

Biological Background: Immunoglobulin heavy constant mu (IgM heavy chain) Function and Localization

• Immunoglobulin heavy constant mu (IgM heavy chain) is the constant region of IgM antibodies, produced by B lymphocytes. It forms the heavy chain of both membrane-bound B cell receptors (BCR) and secreted antibodies, playing a central role in humoral immunity. Related references: PMID:20176268 PMID:22158414 PMID:17576170
• Secreted IgM (sIgM) assembles into pentamers or hexamers, providing multivalent high-avidity antigen binding essential for efficient pathogen neutralization and complement activation. Related references: PMID:32029689 PMID:37095205
• sIgM mediates effector functions through interaction with the high-affinity Fc receptor FCMR on lymphoid cells and recruitment of C1q to trigger the classical complement pathway, facilitating phagocytosis of pathogens and apoptotic cells. Related references: PMID:12847249 PMID:19006321 PMID:28230186
• In mucosal immunity, sIgM is transported across epithelial barriers via the polymeric immunoglobulin receptor (PIGR) and can undergo retrotranscytosis across M cells for antigen presentation. Related references: PMID:32029689
• Membrane-bound IgM (mIgM) is an essential component of the BCR complex, providing constitutive tonic signals necessary for B cell survival and mediating pre-BCR signaling that regulates B cell development and Ig gene rearrangement.
• Subcellular localization: Secreted and cell membrane. Post-translational modifications include disulfide bonds and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 218-453) spans the constant region of human IgM, ensuring recognition of both secreted and membrane-bound forms.
• For flow cytometry, titrate the antibody on B lymphocyte cell lines and PBMCs. Include appropriate isotype controls.
• Consider testing antigen retrieval or permeabilization conditions if intracellular staining is required; note that is a bright fluorophore suitable for multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, offering a comprehensive portfolio of validated antibodies, bulk reagents, and rare-target sourcing. This anti-IgM monoclonal antibody exemplifies our commitment to supporting humoral immunity and B cell research with high-quality tools. From concept to clinic, CamelBio streamlines your development pipeline with integrated technical and consulting services.