Rabbit anti-Human/Monkey IgD Monoclonal Antibody for Flow Cytometry - P01880

Core Product Specifications and Parameters

Biological Background: IgD Function and Localization

• Immunoglobulin heavy constant delta (IgD) is the constant region of the delta heavy chain, forming part of immunoglobulins that mediate the effector phase of humoral immunity by eliminating bound antigens. Related references: PMID:20176268
  PMID:22158414
• The variable domains of IgD are assembled through V(D)J recombination and undergo somatic hypermutation to achieve antigen affinity maturation. Related references: PMID:17576170
  PMID:20176268
• Membrane-bound IgD (mIgD) is the major antigen receptor isotype on most peripheral B-cells, coexpressed with IgM, and induces phosphorylation of CD79A and CD79B upon antigen binding.
• Soluble IgD (sIgD) is present in serum at concentrations lower than IgG, IgA, and IgM but higher than IgE; its secretion is shut off following antigen engagement.
• IgD is a potent inducer of cytokines such as TNF, IL1B, IL1RN, IL6, IL10, and LIF from peripheral blood mononuclear cells, with monocytes being the primary producers in vitro. Related references: PMID:10702483
  PMID:11282392
  PMID:8774350
• IgD exists in two forms: membrane-bound on B-cells and secreted into the extracellular space.
• The IgD heavy chain is a glycoprotein with intrachain and interchain disulfide bonds, crucial for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 99-384 of human IgD, targeting the constant region.
• For flow cytometry, this antibody can be used to detect IgD surface expression on human and cynomolgus B lymphocytes.
• Optimize staining conditions and include appropriate isotype controls.
• Validate the antibody in the specific cell system of interest, as performance may vary with sample preparation and fixation.
• Consider using this antibody in combination with anti-IgM for B-cell subset analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to support diagnostic manufacturers and research laboratories. For IgD-related B-cell and immunology studies, we offer one-stop sourcing from concept to clinic, helping you accelerate assay development and production.