Rabbit anti-Human/Monkey IgD Monoclonal Antibody for Flow Cytometry - P01880

Core Product Specifications and Parameters

Biological Background: IgD Function and Localization

• IgD is the constant region of immunoglobulin heavy chains, serving as the major antigen receptor isotype on most mature peripheral B-cells, where it is co-expressed with IgM. The membrane-bound form (mIgD) triggers B-cell activation via CD79A/CD79B phosphorylation by Src family tyrosine kinases. Related references: PMID:20176268, PMID:22158414

• The variable domains of IgD, assembled through V-(D)-J recombination and somatic hypermutation, pair with light chain variable domains to form two antigen-binding sites, enabling affinity maturation upon antigen exposure. Related references: PMID:17576170, PMID:20176268

• Soluble IgD (sIgD) is secreted into serum at concentrations lower than IgG, IgA, and IgM but higher than IgE; its production is shut off following antigen binding to the B-cell receptor.

• IgD is a potent immunomodulator capable of inducing cytokine release (TNF, IL1B, IL1RN, IL6, IL10, LIF) from peripheral blood mononuclear cells, with monocytes identified as primary responding cells in vitro. Related references: PMID:10702483, PMID:11282392, PMID:8774350

• Subcellularly, IgD exists as both a membrane-bound receptor on B cells and a secreted glycoprotein, consistent with its dual roles in antigen recognition and humoral effector functions.

• The protein is classified under immunoglobulin domain, disulfide bond, glycoprotein, and transmembrane helix keywords, and its UniProt record indicates alternative splicing.

• The predicted molecular weight of the canonical isoform is approximately 47.5 kDa, aligning with the observed 47 kDa band reported for this antibody.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant fusion protein spanning amino acids 99-384 of human IgD, targeting the constant region. It is validated for flow cytometry; ensure appropriate cell permeabilization and staining protocols are optimized for membrane-bound IgD detection.
• For studies involving cynomolgus monkey as a model organism, cross-reactivity has been confirmed; always include isotype controls and single-color compensations when designing multi-color FC panels.
• Consider pairing this labeled anti-IgD antibody with anti-IgM and B-cell lineage markers (e.g., CD19, CD20) to characterize B-cell subpopulations and activation states.
• The immunogen matches the constant domain; therefore, the antibody may recognize both membrane-bound and soluble forms. Validate specificity in your sample system using B-cell lysates or supernatants as appropriate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a one-stop resource for IVD raw materials, technical services, and expert consulting—supporting projects from concept to clinic. Our offering includes validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like IgD. For B-cell research and humoral immunity studies, we help streamline assay development with reliable, high-quality reagents and technical guidance.