Rabbit anti-Human/Monkey IgD Monoclonal Antibody for Flow Cytometry - P01880

Core Product Specifications and Parameters

Biological Background: Immunoglobulin heavy constant delta (IgD) Function and Localization

• IgD is the constant region of immunoglobulin delta heavy chains, forming both membrane-bound antigen receptors on B cells and secreted antibodies.
• Membrane-bound IgD, coexpressed with IgM, serves as the major antigen receptor on mature peripheral B cells, triggering clonal expansion and differentiation upon antigen binding. Related references: PMID:20176268, PMID:22158414
• Variable domains are generated by V-(D)-J rearrangement and somatic hypermutation, allowing affinity maturation for specific antigens. Related references: PMID:17576170, PMID:20176268
• Secreted IgD induces pro-inflammatory cytokine production (TNF, IL1B, IL6, IL10, LIF) from peripheral blood mononuclear cells, primarily monocytes. Related references: PMID:10702483, PMID:11282392, PMID:8774350
• IgD is expressed on the cell membrane and secreted into serum, with soluble IgD levels lower than IgG, IgA, and IgM but higher than IgE.
• Additional keywords: alternative splicing, glycoprotein, disulfide bond, immunoglobulin domain, transmembrane helix, and 3D structures available.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 99-384 of human IgD, targeting the constant region; cross-reactivity with Rhesus monkey suggests conserved epitopes.
• For flow cytometry, this conjugate enables direct detection without secondary staining; consider appropriate controls (e.g., isotype control) to assess specificity.
• Validate antibody performance in your specific sample system (e.g., human peripheral blood B cells, rhesus monkey lymphocytes) and optimize fixation/permeabilization protocols if needed.
• Titrate antibody concentration to achieve optimal signal-to-noise ratio for your experimental setup; typical starting concentrations range from 0.5–5 μg/mL.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents to support IgD research and diagnostic development. From concept to clinic, we provide comprehensive technical services and consulting, ensuring you have access to the precise raw materials you need. Partner with us to streamline your workflow and accelerate the development of high-quality immunoreagents.