Rabbit anti-Human/Monkey HLA-DR Monoclonal Antibody for Flow Cytometry - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DR Alpha Chain Function and Localization

• An alpha chain of major histocompatibility complex class II (MHCII) molecule; pairs with beta chain HLA-DRB to form the antigen-binding complex.
  Related references: PMID:15265931, PMID:15322540, PMID:17334368
• Presents extracellular peptide antigens (10–30 amino acids) derived from endocytosed proteins processed in lysosomes to CD4+ T cells.
  Related references: PMID:8145819
• In the tumor microenvironment, presents peptides from phagocytosed apoptotic tumor cells or macropinocytosis of secreted tumor proteins.
  Related references: PMID:31495665
• Mediates presentation of intracellular self-peptides via macroautophagy, critical for thymic T cell selection and central tolerance.
  Related references: PMID:17182262, PMID:23783831
• Peptide loading follows two processing modes: “bind first, cut/trim later” for exogenous antigens and “cut first, bind later” for self-antigens; anchor residue at peptide position 1 is essential.
  Related references: PMID:25413013, PMID:8145819
• Localized to cell membrane and intracellular compartments: endoplasmic reticulum, early/late endosomes, lysosomes, and autolysosomes.
• Expressed in professional antigen-presenting cells (macrophages, dendritic cells, B cells) and thymic epithelial cells.
  Related references: PMID:15322540, PMID:23783831, PMID:31495665
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination.

Experimental Guidance and Technical Tips

• This -conjugated antibody is directly labeled, eliminating the need for a secondary antibody in flow cytometry; use appropriate filter sets (e.g., 660/20 nm) for detection.
• Titrate the antibody to determine the optimal staining concentration for your specific sample type.
• For human samples, HLA-DR is highly expressed on B cells, monocytes, and dendritic cells in PBMCs; for cynomolgus monkey, validate cross-reactivity with appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement with one-stop access to validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This HLA-DR monoclonal antibody supports immunology and histocompatibility research applications. For custom rare-target sourcing or full technical support from concept to clinic, contact CamelBio.