Rabbit anti-Human/Monkey HLA-DR Monoclonal Antibody for FC - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DR Function and Localization

• HLA-DRA encodes the alpha chain of HLA-DR, an MHC class II molecule that pairs with the beta chain (HLA-DRB) to form a functional heterodimer on antigen-presenting cells.
• The HLA-DR complex presents extracellular peptide antigens (10–30 amino acids) derived from endocytosed proteins to CD4+ T cells, initiating adaptive immune responses. Related references: PMID:8145819 PMID:15265931 PMID:15322540 PMID:17334368
• In the tumor microenvironment, HLA-DR presents tumor-derived peptides captured via phagocytosis of apoptotic cells or macropinocytosis, shaping anti-tumor immunity. Related references: PMID:31495665
• HLA-DR also presents intracellular antigens via autophagy, a pathway critical for thymic T cell selection and central tolerance. Related references: PMID:17182262 PMID:23783831
• Peptide loading onto HLA-DR follows two processing modes: "bind first, cut/trim later" for exogenous antigens and "cut first, bind later" for self-peptides, with a large hydrophobic anchor at peptide position 1 essential for binding. Related references: PMID:25413013 PMID:8145819
• Subcellular localization includes the cell membrane, endoplasmic reticulum membrane, early endosome membrane, late endosome membrane, lysosome membrane, and autolysosome membrane, reflecting its dynamic trafficking to load and present peptide antigens.
• Expressed in professional antigen-presenting cells (macrophages, dendritic cells, B cells) and thymic epithelial cells at the protein level. Related references: PMID:15322540 PMID:23783831 PMID:31495665
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination, which may regulate its stability and function.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for specific cell populations of interest.
• is compatible with violet laser (405 nm) excitation and commonly used 450/50 bandpass filters; ensure instrument configuration matches before acquisition.
• Include appropriate controls such as isotype controls, unstained cells, and fluorescence minus one (FMO) controls to set gates accurately.
• Follow standard surface staining protocols; if fixation/permeabilization is required, verify that the conjugation and epitope recognition are stable under those conditions.
• Recombinant protein-based immunogen suggests recognition of native structure; however, test performance on endogenous protein in relevant sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For HLA-DR and immune profiling applications, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Leverage our expertise to source rare-target raw materials, ensuring your project advances efficiently with reliable supply and expert guidance.