Rabbit anti-Human/Monkey HLA-DR mAb for FC - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DRA Function and Localization

• HLA-DRA encodes the alpha chain of MHC class II molecule HLA-DR, which pairs with beta chain (HLA-DRB) to present antigens to CD4+ T cells, triggering adaptive immune responses including antibody production and macrophage activation.
  Related references:
  PMID:15265931 PMID:15322540 PMID:17334368
• It presents extracellular peptide antigens of 10–30 amino acids generated by lysosomal proteolysis of endocytosed antigens; a large hydrophobic anchor residue at peptide position 1 is critical for stable MHCII binding.
  Related references:
  PMID:8145819
• In tumors, HLA-DR presents antigenic peptides derived from phagocytosed apoptotic tumor cells or macropinocytosis of secreted tumor proteins, shaping anti-tumor immunity.
  Related references:
  PMID:31495665
• Macroautophagy traps intracellular proteins in autolysosomes; their peptides are then presented by HLA-DR, a process important for thymic T cell selection and central tolerance.
  Related references:
  PMID:17182262 PMID:23783831
• Two epitope selection modes operate: “bind first, cut/trim later” for pathogen-derived antigens and “cut first, bind later” for self-peptides, influencing immunodominance.
  Related references:
  PMID:25413013
• Subcellular localization spans the cell membrane, endoplasmic reticulum, early and late endosomes, lysosomes, and autolysosomes, mirroring its dynamic trafficking during antigen processing.
• Tissue expression is restricted to professional antigen-presenting cells (macrophages, dendritic cells, B cells) and thymic epithelial cells.
• Post‑translational modifications include N‑linked glycosylation, disulfide bonds, isopeptide bonds, and ubiquitin‑like conjugation, which modulate MHCII stability and peptide loading.

Experimental Guidance and Technical Tips

• is a bright, photostable fluorophore directly conjugated to the antibody; no secondary detection reagent is needed, minimizing non‑specific background in flow cytometry.
• This monoclonal rabbit antibody targets the nearly invariant HLA‑DRA chain, making it a pan‑HLA‑DR marker for human and cynomolgus monkey immune cells.
• For optimal staining, titrate the antibody in the relevant cell type to determine the concentration that gives maximal separation between positive and negative populations. Include appropriate isotype controls and Fc block if needed.
• Validate the staining panel with single‑color controls and fluorescence minus one (FMO) controls to ensure accurate compensation and gating, especially in multicolor experiments.
• Consider fixing cells after staining with a compatible fixative to preserve fluorescence and sample stability for later analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive inventory of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. When you need rare‑target monoclonal antibodies or bulk orders of conjugated flow cytometry reagents like this anti‑HLA‑DR mAb, we streamline procurement and provide technical consulting to accelerate your immunology or immuno‑oncology assay development. From single antibodies to full workflow solutions, CamelBio bridges concept to clinical application.