Rabbit anti-Human/Monkey HLA-DR mAb for FC - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DRA Function and Localization

• HLA-DRA encodes the alpha chain of MHC class II molecules, which heterodimerize with beta chains (e.g., HLA-DRB) to present antigenic peptides to CD4+ T cells, driving T-helper–mediated antibody responses and macrophage activation for pathogen and tumor clearance. Related references: PMID:15265931 PMID:15322540 PMID:17334368
• The MHCII complex typically binds extracellular peptide antigens of 10–30 amino acids that are generated by proteolysis of endocytosed antigens in lysosomes. Related references: PMID:8145819
• In the tumor microenvironment, HLA-DR presents antigenic peptides derived from phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins by resident APCs, contributing to anti-tumor immunity. Related references: PMID:31495665
• Intracellular antigens trapped in autolysosomes after macroautophagy can also be presented by MHCII, a process critical for T cell selection in the thymus and central immune tolerance. Related references: PMID:17182262 PMID:23783831
• Selection of immunodominant epitopes follows two processing modes: “bind first, cut/trim later” for pathogen-derived peptides and “cut first, bind later” for self-peptides, with a large hydrophobic anchor residue at peptide position 1 being essential for high-affinity MHCII interaction. Related references: PMID:25413013 PMID:8145819
• Subcellular localization includes the cell membrane and intracellular compartments such as the endoplasmic reticulum, early and late endosomes, lysosomes, and autolysosomes, reflecting its trafficking through the antigen-processing pathway.
• Tissue expression is restricted to professional antigen-presenting cells (macrophages, dendritic cells, B cells) and thymic epithelial cells, as confirmed at the protein level. Related references: PMID:15322540 PMID:23783831 PMID:31495665

Experimental Guidance and Technical Tips

• The immunogen is a recombinant full-length human HLA-DRA protein; the antibody may recognize native conformation and linear epitopes.
• For flow cytometry, use standard staining protocols with appropriate isotype controls and viability dyes.
• Consider performing an antibody titration to determine the optimal staining index for your specific sample type.
• Cross-reactivity with cynomolgus monkey enables translational studies in non-human primate models; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials for immune monitoring and oncology applications, with this anti-HLA-DR monoclonal antibody as part of our validated portfolio. Our supply capabilities include optimized antibody pairs, multiple clone options for MHC class II targets, and bulk ancillary reagents to accelerate assay development. Our global sourcing network supports rare target and custom raw-material procurement, helping you advance projects from concept to clinic with reliable, scalable supply.