Rabbit anti-Human/Monkey CD8a Monoclonal Antibody for Flow Cytometry - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8A is T-cell surface glycoprotein CD8 alpha chain, also known as T8/Leu-2, encoded by the CD8A gene in humans. Alternative splicing produces multiple isoforms.
• Its core function is as an integral membrane glycoprotein coreceptor for MHC class I molecules. In T-cells, it interacts simultaneously with the T-cell receptor (TCR) and MHC class I:peptide complex, recruiting the Src kinase LCK to the TCR-CD3 complex to initiate intracellular signaling pathways leading to cytotoxic T-lymphocyte (CTL) activation, lymphokine production, and adhesion.
• CD8A homodimers on natural killer (NK) cells provide a survival mechanism, enabling conjugation and lysis of multiple target cells, and promote the differentiation of activated lymphocytes into memory CD8 T-cells.
• CD8A belongs to the immunoglobulin superfamily and typically forms disulfide-linked heterodimers with CD8B (CD8 beta chain) on thymus-derived T-cells; homodimers are also expressed on subsets of immune cells.
• Subcellular localization includes the cell membrane and secreted form. It is a single-pass type I membrane protein with a signal peptide, an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail.
• Tissue specificity: Expressed on cytotoxic T-cells, a subset of NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, dendritic cells, and on plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.
• Post-translational modifications and keywords: Glycosylated, palmitoylated, and contains disulfide bonds. Annotated as a glycoprotein, lipoprotein, disulfide bond; involved in adaptive immunity, alternative splicing; linked to disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the extracellular region (aa 22-182). This design likely yields antibodies that recognize surface epitopes on live cells without permeabilization, making it well-suited for direct flow cytometry (FC) staining.
• For FC, titrate the conjugate to determine the optimal working dilution. Include appropriate isotype control antibodies and Fc receptor blocking. Verify specificity using known CD8A-positive cell lines (e.g., Jurkat) or peripheral blood mononuclear cells.
• Cross-reactivity is reported with cynomolgus monkey. If using cynomolgus samples, validate the antibody in your own assay system with proper controls.
• Multiple protein weights (21, 25, 30 kDa) are noted; these likely reflect differentially glycosylated forms or isoform variants. If performing western blot as a confirmatory step, expect potential multiple bands.
• For multicolor flow cytometry panels, confirm minimal spectral overlap with other fluorophores and include compensation controls. The rabbit monoclonal nature and direct conjugation facilitate reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner dedicated to aiding diagnostic manufacturers and research laboratories with validated biological reagents. This anti-CD8a monoclonal antibody, optimized for immune cell profiling, illustrates our ability to supply pre-conjugated flow cytometry antibodies, bulk ancillary reagents, and custom antigens. Whether you require validated antibody pairs, monoclonal/polyclonal antibodies for special targets, or rare target raw-material sourcing, CamelBio is committed to streamlining your development pipeline from concept to clinic.