Rabbit anti-Human/Monkey CD8a Monoclonal Antibody for FC - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8A encodes the alpha chain of the T-cell surface glycoprotein CD8, also known as T8/Leu-2, a coreceptor critical for adaptive immunity.
• Functions as a coreceptor for MHC class I molecules, interacting simultaneously with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells to facilitate recognition of cytosolic antigens.
• Recruits Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that lead to T-cell activation, lymphokine production, motility, adhesion, and cytotoxic T-lymphocyte (CTL)-mediated killing of infected and tumor cells.
• In NK cells, CD8A homodimers provide a survival mechanism enabling conjugation and lysis of multiple target cells, and also promote differentiation of activated lymphocytes into memory CD8 T-cells.
• Expressed as heterodimers with CD8B on thymus-derived T-cells, but can also form homodimers on subsets of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• Subcellular localization is primarily the cell membrane, but a secreted form also exists.
• Post-translational modifications include disulfide bonds, glycosylation, palmitoylation, and proteolytic cleavage of the signal peptide.
• Alternative splicing generates multiple isoforms with distinct properties.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC); optimize antibody titration (e.g., 0.5–5 µg per 10⁶ cells) and use appropriate isotype controls.
• Since the immunogen corresponds to the extracellular domain (aa 22–182), the antibody likely recognizes an extracellular epitope, suitable for surface staining without permeabilization.
• Cross-reactivity with cynomolgus monkey CD8a expands utility to non-human primate models; validate in the relevant sample system.
• Avoid repeated freeze-thaw cycles; aliquot and store at recommended conditions (ice bag shipping suggests short-term 2–8°C, long-term –20°C; check manufacturer's instructions).
• For multicolor FC, confirm minimal spectral overlap with other fluorophores in the panel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents to support diagnostic development from concept to clinic. This anti-CD8a antibody addresses immunology and T-cell biology research needs, and CamelBio can assist with bulk supply, custom conjugation, and rare target sourcing. Partner with us to streamline your supply chain and accelerate your immunoassay development.