Rabbit anti-Human/Monkey CD8a mAb - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a is a T-cell surface glycoprotein, also known as T-lymphocyte differentiation antigen T8/Leu-2, encoded by the CD8A gene.
• It plays a critical role in adaptive immunity as a coreceptor for MHC class I molecules, interacting with the T-cell receptor (TCR) and MHC I-peptide complexes on antigen-presenting cells.
• The interaction recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling that leads to cytotoxic T-lymphocyte activation, proliferation, and target cell killing.
• In natural killer (NK) cells, CD8A homodimers promote survival and enhance conjugation and lysis of target cells; homodimers also support differentiation of activated lymphocytes into memory CD8 T-cells.
• The protein contains an immunoglobulin domain and undergoes post-translational modifications including disulfide bond formation, glycosylation, and palmitoylation.
• CD8A is predominantly expressed at the cell membrane of thymus-derived T-cells, usually as a heterodimer with CD8B, but homodimers are found on NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• Expressed on plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-182), targeting an exposed epitope suitable for live-cell flow cytometry.
• Titrate the antibody to determine the optimal concentration for your specific cell population and staining conditions.
• As a direct conjugate with , it is compatible with multicolor flow cytometry panels; consider compensation controls and spectral overlap.
• Validate performance in your experimental system using appropriate positive (e.g., human PBMCs) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research institutions with reliable access to IVD raw materials, including validated monoclonal antibodies like this anti-CD8a clone for immunophenotyping applications. Our platform streamlines procurement of antibody pairs, bulk ancillary reagents, and rare-target raw materials, helping you advance T-cell biology and immuno-oncology projects from concept to clinic.