Rabbit anti-Human/Monkey CD86 Monoclonal Antibody for Flow Cytometry - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86, also known as B7-2, B70, or CD28LG2, is a type I membrane glycoprotein and a member of the immunoglobulin superfamily, functioning as a costimulatory molecule for T-lymphocyte activation.
• It serves as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by binding to the CD28 receptor, which is constitutively expressed on T-cells, playing a critical role in the decision between immunity and anergy within 24 hours of activation. Related references: PMID:12196291, PMID:7694363, PMID:7527824
• CD86 also acts as an inhibitor of T-cell activation by engaging CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming. Related references: PMID:11279501
• Upon CD40 engagement, CD86 activates the NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.
• It interferes with the formation of CD86 clusters, acting as a negative regulator of T-cell activation.
• CD86 acts as a receptor for adenovirus subgroup B, mediating viral entry.
• Subcellularly, CD86 localizes to the cell membrane.
• It is specifically expressed on the surfaces of antigen-presenting cells (APCs), including dendritic cells, macrophages, and B cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-241 of human CD86, representing the extracellular domain; this region is involved in ligand binding and is well-conserved, supporting the observed cross-reactivity with cynomolgus monkey.
• This antibody is directly conjugated to , a bright and photostable fluorophore, making it suitable for multi-color flow cytometry panels. It is recommended to titrate the antibody for optimal staining concentration in the relevant cell system.
• As CD86 is expressed on activated antigen-presenting cells, appropriate positive controls (e.g., stimulated dendritic cells or B cells) and Fc receptor blocking steps should be considered.
• For best results, validate antibody performance in your specific experimental context, as staining intensity may vary with cell type and activation state.

CamelBio: Your One-Stop Sourcing Bridge

With extensive experience in IVD raw material supply, CamelBio provides a one-stop sourcing bridge, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic developers and research laboratories. This anti-CD86 monoclonal antibody supports immunology research and T-cell activation studies, and we can assist with rare target raw-material sourcing and technical consulting to accelerate your assay development from concept to clinic.