Rabbit anti-Human/Monkey CD86 Monoclonal Antibody for FC - P42081

Core Product Specifications and Parameters

Biological Background: CD86 Function and Localization

• CD86 (B7-2) is a costimulatory molecule of the immunoglobulin superfamily essential for T-lymphocyte activation. Related references: PMID:12196291, PMID:7694363
• It serves as the primary ligand for CD28, augmenting MHC/TCR signaling in naive T-cells. Related references: PMID:12196291, PMID:7694363
• CD86 also binds CTLA-4, acting as an inhibitor of T-cell activation by blocking CD28-mediated priming. Related references: PMID:11279501
• May influence early T-cell activation decisions, such as immunity versus anergy, within 24 hours after activation. Related references: PMID:7527824
• Additionally, CD86 regulates B cell function; it modulates IgG1 production and activates NF-kappa-B signaling upon CD40 engagement (By similarity).
• The protein is localized at the cell membrane and expressed on the surfaces of antigen-presenting cells.
• It also acts as a receptor for adenovirus subgroup B, mediating viral entry.
• CD86 is a glycosylated transmembrane protein with immunoglobulin domains, disulfide bonds, and undergoes ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 20-241), designed to target the native conformation of cell surface CD86.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your cell model; include appropriate isotype and fluorescence-minus-one (FMO) controls.
• has excitation/emission peaks ~590/617 nm; ensure instrument compatibility and avoid fixation methods that may alter epitope recognition.
• With confirmed cross-reactivity to cynomolgus monkey, this antibody may be suitable for non-human primate studies; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers a one-stop sourcing solution for IVD raw materials, from concept to clinic. This CD86 monoclonal antibody exemplifies our portfolio of validated immunological reagents, and we can supply matched antibody pairs, bulk ancillary reagents, and undertake rare-target sourcing to accelerate your assay development and manufacturing.