Rabbit anti-Human/Monkey CD69 Monoclonal Antibody for Flow Cytometry - Q07108

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 (Early activation antigen CD69; also known as AIM, EA1, CLEC2C, GP32/28, Leu-23) is a C-type lectin transmembrane protein encoded by the CD69 gene.
• It plays a critical role in immune cell homeostasis, as it is rapidly expressed on the surface of platelets, T-lymphocytes, and NK cells upon activation by antigens or cytokines, stimulating different signaling pathways in various cell types (PubMed:24752896, PubMed:26296369, PubMed:35930205). Related references: PMID:24752896 PMID:26296369 PMID:35930205
• CD69 negatively regulates Th17 cell differentiation via carbohydrate-dependent interaction with galectin-1/LGALS1 on immature dendritic cells (PubMed:24752896). Related references: PMID:24752896
• CD69 promotes the conversion of naive CD4 T-cells into regulatory T-cells via the S100A8/S100A9 complex present on PBMCs (PubMed:26296369). Related references: PMID:26296369
• Acting as an oxidized low-density lipoprotein (oxLDL) receptor on CD4 T-lymphocytes, CD69 negatively regulates inflammation by inducing PDCD1 expression through NFAT activation (PubMed:35930205). Related references: PMID:35930205
• CD69 participates in protection against Pseudomonas aeruginosa infection by recognizing the pathogen and promoting ERK1 activation, leading to GM-CSF and inflammatory cytokine secretion (PubMed:34841721). Related references: PMID:34841721
• CD69 negatively regulates chemotactic responses of effector lymphocytes and dendritic cells to sphingosine 1-phosphate (S1P) by acting as an S1PR1 agonist and facilitating receptor internalization and degradation (PubMed:37039481). Related references: PMID:37039481
• Subcellularly, CD69 is a cell membrane protein; it is expressed on activated T-cells, B-cells, NK cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets. The protein is a glycoprotein, phosphoprotein, and contains a disulfide bond, with a molecular weight of approximately 22.6 kDa (UniProt: Q07108).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 78–199) of human CD69, targeting the surface-exposed domain for flow cytometry detection.
• As a directly conjugated antibody, it enables one-step staining, reducing background and simplifying multicolor panel design.
• When implementing flow cytometry assays, consider titrating the antibody to determine the optimal concentration for your specific cell type and activation conditions.
• Include appropriate isotype controls and compensation controls to ensure specificity and accurate gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw materials, providing diagnostic manufacturers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For immune cell activation studies and flow cytometry applications, we offer a wide range of CD69 and related immunological reagents. Our supply capabilities help streamline assay development from concept to clinic, supporting your research and diagnostic needs with reliable and efficient sourcing solutions.