Rabbit anti-Human/Monkey CD64 mAb - P12314

Core Product Specifications and Parameters

Biological Background: CD64 Function and Localization

• CD64 is the high-affinity receptor for the Fc region of immunoglobulin gamma (IgG), bridging humoral and cellular immunity.
• It functions in both innate and adaptive immune responses, mediating IgG effector functions primarily on monocytes and macrophages.
• Upon engagement, CD64 triggers antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells.
• Encoded by the FCGR1A gene, the protein is a transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• Subcellular localization is restricted to the cell membrane.
• Tissue expression is monocyte/macrophage specific, consistent with its role in professional phagocytes.
• Post-translational modifications include N-glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 16–288), suggesting suitability for detecting cell surface CD64 by flow cytometry.
• For FC applications, consider titrating the antibody on relevant monocyte or macrophage populations to determine optimal concentration.
• Given the cross-reactivity with Cynomolgus monkey, validate staining in parallel with human and non-human primate samples if applicable.
• Include appropriate isotype controls and Fc blocking reagents to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this rabbit anti-human/monkey CD64 monoclonal antibody as part of a comprehensive IVD raw-material portfolio, supporting manufacturers in developing immune monitoring and ADCC assays. From validated antibody pairs to bulk ancillary reagents, CamelBio streamlines procurement for diagnostic development—backed by one-stop technical services and consulting from concept to clinic. If your project requires rare-target reagents or customized raw-material sourcing, our team is ready to assist.