Rabbit anti-Human/Monkey CD64 mAb for Flow Cytometry - P12314

Core Product Specifications and Parameters

Biological Background: CD64 (FcγRI) Function and Localization

• CD64, also known as high affinity immunoglobulin gamma Fc receptor I (FcγRI), is a key membrane receptor that binds the Fc region of IgG antibodies with high affinity. It is encoded by the FCGR1A gene in humans and is also referred to as FCG1, FCRI, or IGFR1.
• Functionally, CD64 plays a pivotal role in both innate and adaptive immunity by mediating IgG effector functions, including the activation of antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells on monocytes and macrophages.
• As a member of the immunoglobulin superfamily, CD64 contains extracellular immunoglobulin-like domains that facilitate specific IgG binding, contributing to the engulfment and clearance of immune complexes.
• The receptor is predominantly expressed on the cell membrane of monocytes and macrophages, with tissue specificity restricted to these phagocytic lineages, making it a reliable marker for these cell populations.
• Post-translational modifications of CD64 include glycosylation, phosphorylation, and disulfide bond formation, which are important for receptor stability, ligand binding, and intracellular signaling. The protein undergoes alternative splicing, generating isoforms with potential functional diversity.
• CD64 expression is upregulated during infections and inflammatory conditions, reflecting its critical involvement in immune surveillance and response, and it has been implicated in various autoimmune and inflammatory disorders.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody spans amino acids 16–288, which corresponds to the extracellular domain of CD64. This design suggests the antibody may be effective in detecting the native cell-surface receptor in flow cytometry applications.
• For flow cytometry experiments, consider titrating the antibody to determine the optimal staining concentration for your specific sample type (e.g., human whole blood or isolated PBMCs), as staining efficiency can vary.
• Given the monocyte/macrophage-restricted expression of CD64, validate staining specificity by including appropriate controls such as isotype controls, Fc blocking reagents, and comparison with known monocyte markers like CD14.
• When working with cynomolgus monkey samples, verify cross-reactivity under your experimental conditions, even though the antibody is reported to react, as epitope conservation may differ slightly from human.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports diagnostic developers and researchers by supplying validated antibodies, recombinant proteins, and related reagents for immune-targeted assays. This anti-CD64 monoclonal antibody facilitates the detection of FcγRI-positive cells in flow cytometry, and CamelBio can complement it with optimized ancillary reagents, bulk antibody orders, and rare target sourcing. For projects requiring robust immune cell profiling or antibody-dependent functional studies, CamelBio’s integrated procurement solutions simplify your supply chain from discovery to clinical application.