Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA), is a receptor-type tyrosine-protein phosphatase encoded by the PTPRC gene in humans.
• It is a transmembrane glycoprotein expressed on all nucleated hematopoietic cells and exists in several isoforms generated by alternative splicing, resulting in molecular weights ranging from 131 kDa to 147 kDa.
• CD45 plays a critical role in T-cell activation by dephosphorylating key signaling molecules such as LYN, FYN, and SKAP1, thereby modulating T-cell receptor signaling thresholds. Related references: PMID:35767951
• The protein contains two intracellular phosphatase domains; the first (D1) is enzymatically active, while the second (D2) regulates substrate specificity.
• CD45 localizes to the cell membrane and is enriched in membrane rafts at the immunological synapse during antigen-presenting cell–T cell interactions.
• It acts as a receptor for human cytomegalovirus protein UL11, which impairs T-cell proliferation upon binding.
• Tissue specificity: Multiple isoforms are detected in thymocytes, with isoforms 1, 2, 3, 5, and 7 present, while isoforms 4, 6, and 8 are not detected.
• Keywords: Alternative splicing, Glycoprotein, Host-virus interaction, Hydrolase, Membrane, Phosphoprotein, Protein phosphatase, Signal, Synapse, Transmembrane.

Experimental Guidance and Technical Tips

• This -conjugated antibody is intended for flow cytometry; optimize titration and include appropriate isotype controls in experiments.
• Protect the conjugate from prolonged light exposure to maintain fluorophore stability.
• Validate staining in the relevant sample system, such as PBMCs from human or cynomolgus monkey donors.
• The immunogen spans the extracellular domain (aa 26-577); the antibody may recognize multiple CD45 isoforms.
• Performance in other applications (e.g., WB, IHC) has not been determined; if required, conduct pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive IVD raw material sourcing solutions. For immunology research and flow cytometry applications like CD45 detection, we offer validated monoclonal antibodies, bulk reagents, and flexible access to rare-target raw materials. Partner with us to streamline your procurement from concept to clinic.