Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575-9

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) and T200, is a receptor-type tyrosine-protein phosphatase encoded by the PTPRC gene.
• It functions as a key regulator of protein tyrosine phosphorylation in immune cells, modulating signal transduction pathways.
• This antibody recognizes isoform 7 of CD45 (UniProt P08575-9), which has a predicted molecular weight of 135.7 kDa.
• CD45 is widely expressed on all nucleated hematopoietic cells, serving as a major leukocyte surface marker for identification and isolation.
• The multiple aliases of CD45 (e.g., B220, CD45R, GP180) reflect its recognition by various monoclonal antibodies and its central role in immunological research.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate this -conjugated antibody on your target cells to determine the optimal staining concentration for your specific experimental conditions.
• The immunogen (amino acids 26-577 of human CD45) covers a large extracellular region; verify that the epitope is preserved under your fixation and permeabilization protocol if intracellular staining is needed.
• Since CD45 is highly expressed on leukocytes, this antibody can be effectively used in multicolor panels combined with lineage-specific markers to distinguish lymphocyte subsets.
• The reported cross-reactivity with cynomolgus monkey suggests potential utility in non-human primate studies; validation in your specific monkey sample type is recommended.
• Store and handle the antibody according to the ice bag shipping condition; avoid repeated freeze-thaw cycles to maintain conjugate stability and fluorescence intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this validated CD45 monoclonal antibody for flow cytometry applications. From monoclonal antibodies to ancillary reagents and bulk supplies, we support your assay development and production needs with a streamlined supply chain and technical consultation. Whether you require routine catalog reagents or custom-sourced rare targets, CamelBio offers end-to-end procurement solutions to bridge the gap from concept to clinic.