Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575-8

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 is a receptor-type tyrosine-protein phosphatase, encoded by the PTPRC gene.
• Alternative names include leukocyte common antigen (L-CA), T200, and several CD designations (LCA, LY5, B220, CD45R).
• The leukocyte common antigen alias reflects its widespread expression on the surface of leukocytes.
• This antibody recognizes isoform 6 (UniProt: P08575-8), which has a predicted molecular weight of approximately 138 kDa.
• CD45 plays a central role in modulating immune cell signaling through its phosphatase activity.

Experimental Guidance and Technical Tips

• Optimize working concentration by titrating the antibody over a recommended range (e.g., 0.1–2 µg per test) in flow cytometry.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to set gating boundaries.
• Validate performance using known CD45-expressing positive controls, such as human PBMCs or cynomolgus monkey lymphocytes.
• Ensure single-cell suspensions and use Fc receptor blocking steps to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to deliver comprehensive IVD raw-material solutions, covering the entire development path from concept to clinic. For immune cell profiling and phenotyping studies, we offer validated anti-CD45 antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare target raw materials, simplifying your procurement and accelerating assay development.