Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (PTPRC), also known as leukocyte common antigen (L-CA) and T200, is a receptor-type tyrosine-protein phosphatase essential for T-cell activation through the antigen receptor. Related references: PMID:35767951
• It positively regulates T-cell coactivation upon binding to DPP4, and modulates downstream signaling by dephosphorylating Src family kinases, including LYN and FYN.
• The intracellular region contains two tandem phosphatase domains; the first domain possesses catalytic activity, while the second influences substrate specificity.
• CD45 interacts with CLEC10A on immature dendritic cells, recognizing Tn antigen-modified CD45 on effector T cells to modulate the activation threshold and limit autoreactivity.
• During human cytomegalovirus infection, CD45 functions as a receptor for viral protein UL11, leading to reduced T-cell receptor signaling and impaired proliferation.
• The protein localizes to the cell membrane, membrane rafts, and the immunological synapse, consistent with its role in antigen receptor signaling.
• Multiple alternatively spliced isoforms exist with cell-type-specific expression patterns; for example, isoform 1 is detected in thymocytes.
• Post-translational modifications include glycosylation and phosphorylation; mutations in PTPRC are associated with severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 26–577), suggesting that this antibody may recognize all CD45 isoforms; however, isoform specificity should be validated in the relevant sample system.
• Designed for flow cytometry; consider including appropriate isotype controls and employing Fc receptor blocking strategies to minimize non-specific binding.
• Given the cross-reactivity with cynomolgus CD45, this antibody can be considered for non-human primate studies; optimal dilution must be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies (like this CD45 clone), and bulk ancillary reagents. For immunology and leukocyte phenotyping applications, we support sourcing needs from concept to clinic, offering rare target raw-material procurement and technical consultation. Contact us to discuss customized antibody development and supply solutions tailored to your immunoassay platform.