Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• Receptor-type tyrosine-protein phosphatase C (CD45), encoded by the PTPRC gene, is also known as leukocyte common antigen (L-CA) and T200. Multiple isoforms exist due to alternative splicing.
• Essential for T-cell activation through the antigen receptor; the first PTPase domain has enzymatic activity, while the second modulates substrate specificity. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN, and modulates LYN activity. Related references: PMID:35767951
• Acts as a receptor for human cytomegalovirus protein UL11, mediating viral binding to T-cells and impairing T-cell proliferation and signaling.
• Localizes to the cell membrane, membrane rafts, and immunological synapse, positioning it at sites of antigen recognition and signal transduction.
• Isoforms 1–3, 5, and 7 are detected in thymocytes, while isoforms 4, 6, and 8 are not, indicating tissue-specific expression patterns.
• Post-translationally modified by glycosylation and phosphorylation; classified under hydrolase, protein phosphatase, glycoprotein, and host-virus interaction keywords.
• Belongs to the protein‑tyrosine phosphatase family, receptor class 1/6 subfamily, acting as a key signaling hub in immune cells.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 26–577 of human CD45, encompassing the extracellular region; thus, the antibody is expected to recognize native extracellular epitopes.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and instrument settings; use an appropriate isotype control.
• Validate staining on known CD45‑positive cells (e.g., human PBMCs, Jurkat) and negative cells (e.g., erythrocytes).
• Because CD45 is highly expressed on leukocytes, consider Fc receptor blocking to minimize nonspecific binding.
• The conjugate is compatible with common emission filters (e.g., 614/20 nm); ensure proper compensation in multicolor panels.
• Fixation and permeabilization may alter CD45 epitope recognition; test whether surface staining is sufficient for your protocol.
• Store undiluted antibody at 2–8°C, protected from light, and avoid repeated freeze‑thaw cycles to maintain conjugate integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This CD45 monoclonal antibody, a key reagent for leukocyte immunophenotyping, exemplifies our ability to supply validated antibody pairs, conjugated specificities, and bulk ancillary reagents. From concept to clinic, CamelBio streamlines sourcing—even for rare targets—ensuring reliable and scalable raw‑material access.