Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for FC - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) and T200, is a receptor-type tyrosine-protein phosphatase encoded by the PTPRC gene.
• It plays a critical role in T-cell activation through the antigen receptor, acting as a positive regulator upon binding to DPP4.
  Related references: PMID:35767951
• The protein contains two phosphatase domains; the first has enzymatic activity while the second influences substrate specificity.
• CD45 localizes to the cell membrane, membrane rafts, and immunological synapse, facilitating signal transduction.
• Multiple isoforms exist due to alternative splicing, with distinct expression patterns; for example, isoforms 1-3 and 5-7 are detected in thymocytes.
• It undergoes post-translational modifications including glycosylation and phosphorylation, as indicated by keywords.
• CD45 also functions as a receptor for human cytomegalovirus protein UL11, leading to impaired T-cell proliferation upon infection.
• It interacts with molecules such as CLEC10A on dendritic cells, modulating T-cell activation threshold to limit autoreactivity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 26-577 of human CD45, targeting the extracellular domain, which is ideal for surface staining in live-cell flow cytometry.
• For FC applications, consider titrating the antibody and including appropriate isotype controls to optimize signal-to-noise ratios.
• Cross-reactivity with cynomolgus CD45 is reported; validate reactivity in your specific sample system if using non-human primate samples.
• When combining with intracellular staining protocols, verify that permeabilization conditions do not affect antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This -conjugated CD45 monoclonal antibody supports immunophenotyping and immune cell analysis in flow cytometry. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your development workflow.