Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for FC - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (LCA) or PTPRC, is a receptor-type tyrosine-protein phosphatase and a major cell surface marker expressed on all nucleated hematopoietic cells.
• It plays a critical role in T-cell activation by dephosphorylating key signaling proteins such as LYN, SKAP1, and FYN upon T-cell receptor stimulation. Related references: PMID:35767951
• The protein has two intracellular phosphatase domains: the first has enzymatic activity, and the second modulates substrate specificity, contributing to its regulatory role in immune cell signaling.
• CD45 interacts with DPP4 and CLEC10A at the immunological synapse, modulating T-cell coactivation and tolerance to prevent autoimmunity.
• It localizes to the cell membrane, membrane rafts, and the immunological synapse, positioning it for rapid antigen receptor signaling.
• Multiple isoforms (1–7) are generated by alternative splicing, with differential expression in thymocytes; the antibody recognizes the common extracellular domain and thus reacts with all isoforms.
• CD45 is heavily glycosylated; the predicted molecular weight is ~147 kDa, though it migrates at approximately 138 kDa (consistent with supplier data) due to extensive glycosylation.
• It serves as a receptor for human cytomegalovirus (HCMV) protein UL11, leading to impaired T-cell proliferation, and mutations in the gene are associated with severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 26–577 of human CD45 (the extracellular domain), indicating the antibody likely targets an epitope exposed on the cell surface, ideal for live-cell staining applications.
• This product is validated for flow cytometry (FC); users should follow standard FC protocols and use appropriate single-stain compensation controls when combining with other fluorophores, as emits in the green channel.
• For other applications (e.g., WB, IHC, IP), performance has not been specified by the supplier; consider in-house validation in relevant sample systems before use.
• Given CD45's high expression on leukocytes, titrate the antibody to optimize signal-to-noise ratio in your experimental setting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibodies, receptors, and ancillary reagents to diagnostic manufacturers and research laboratories. This anti-CD45 monoclonal antibody targets a foundational immunology marker, facilitating immune monitoring and diagnostic assay development. For project-specific needs—including bulk orders, customized conjugates, or technical consulting—CamelBio bridges the gap between early-stage research and clinical application.