Rabbit anti-Human/Monkey CD45 mAb for Flow Cytometry - P08575-9

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 is the gene product of the PTPRC gene and is also known as receptor-type tyrosine-protein phosphatase C.
• The antibody targets isoform 7 (UniProt accession P08575-9), which has a calculated molecular weight of approximately 135.7 kDa.
• CD45 is commonly referred to as leukocyte common antigen (L-CA) or T200 and serves as a pan-leukocyte marker.
• It functions as a receptor-type tyrosine-protein phosphatase, implying a role in cell signaling regulation.
• Multiple isoforms are generated by alternative splicing, with isoform 7 being one variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 26–577 of human CD45 (P08575), covering the extracellular domain and likely encompassing multiple isoforms.
• For flow cytometry, we recommend titrating the antibody to determine the optimal staining concentration for your specific cell type.
• Since the antibody cross-reacts with cynomolgus CD45, it can be utilized for immunophenotyping in non-human primate studies.
• Validate the antibody’s performance in your relevant sample system under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing solutions for IVD manufacturers, offering a wide range of high-quality antibodies and ancillary reagents. For CD45-targeted flow cytometry assays, we can supply validated monoclonal antibodies, including fluorophore-conjugated formats, along with bulk ancillary reagents. Our one-stop platform helps streamline your raw material procurement from discovery to commercialization.