Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a 51 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, expressed on the surface of T-helper cells, macrophages, dendritic cells, and NK cells as a hallmark of helper T lymphocytes.
• As a coreceptor, CD4 binds to MHC class II molecules on antigen-presenting cells, simultaneously interacting with the T-cell receptor (TCR) to recruit the Src kinase LCK, which initiates signaling cascades leading to T-cell activation, cytokine production, and differentiation.
• In the thymus, CD4 participates in the positive selection and lineage commitment of CD4+ T-helper cells, essential for adaptive immunity.
• CD4 serves as the primary receptor for HIV-1, mediating viral entry into host cells; it is subsequently down-regulated by the HIV-1 accessory protein Vpu to aid immune evasion. Related references: PMID:12089508, PMID:16331979, PMID:2214026, PMID:17346169
• CD4 also acts as a cellular receptor for human herpesvirus 7 (HHV-7) entry. Related references: PMID:7909607
• Post-translational modifications include N-linked glycosylation, disulfide bond formation in its extracellular Ig-like domains, and palmitoylation at a cytoplasmic cysteine residue, which modulates membrane localization and signaling.
• The protein localizes predominantly to the plasma membrane via a single-pass transmembrane helix, with its extracellular region organized into four immunoglobulin-like domains (D1–D4) critical for ligand interactions.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant human CD4 protein; therefore, the antibody recognizes native conformation-dependent epitopes and is suitable for surface staining in flow cytometry.
• For FC applications, titrate the antibody using human or cynomolgus peripheral blood mononuclear cells (PBMCs) to determine optimal concentration. Include appropriate isotype controls and perform Fc receptor blocking if necessary.
• Whole blood staining or staining of lymphoid tissue single-cell suspensions may be considered, following standard protocols with appropriate anticoagulants and viability dyes.
• Fixation and permeabilization steps should be validated as they may alter epitope accessibility; for intracellular staining, use protocols optimized for membranous antigens.
• Ensure instrument compensation settings are correctly calibrated for fluorophore to avoid spectral overlap, particularly in multi-color panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop solution for IVD raw materials, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. This anti-CD4 antibody supports immunophenotyping and immune monitoring applications, while our sourcing platform can also supply rare-target raw materials and comprehensive technical support tailored to your development needs. From concept to clinic, CamelBio bridges the gap between discovery and diagnostic commercialization.